Klapperich Lab:Notebook/Lab Meeting Notes/2009/06/02: Difference between revisions

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† Announcements<br>
† Announcements<br>
* SIGN UP FOR NEW LAB DUTIES WITH SONALI.<br>
* SIGN UP FOR NEW LAB DUTIES WITH SONALI.<br>
* Vacuum chamber needs a new gasket. <br>
* Short small team meetings. 45 min each week? <br>
* MicroTAS Abstracts accepted. Papers due June 30th. <br>
Suggested groups... please edit. Teams<br>
1. PCR= QQ, MCK, MM <br>
2. Bacteria = JZ, JD <br>
3. Flu = MM, JZ, HM, JC, BC <br>
3. Lysis? = AG, HM? <br>
* MicroTAS Abstracts accepted. (4) Papers due June 30th. <br>
<br>
<br>
† Flu R01 <br>
† Flu R01 <br>
* RNA extraction troubleshooting. <br>
* RNA extraction troubleshooting. <br>
* Mass Spec of elution? In Chemistry.- We should approach them. <br>
* Bioanalyzer not working.. why? LOD - Serially dilute our samples and look to see if we can see it when we dilute the contaminants and the DNA/RNA: DID NOT SEE ANYTHING. <br>
* Absolute Quantification assays: TH this week. <br>
* Absolute Quantification assays: TH this week. <br>
* FTIR-ATR look at the eluant? There are some organics in the eluant. Restrict the range. Align the laser. <br>
* FTIR <br>
* <br>
* <br>
† Coulter Flu Fraunhofer Project
† Coulter Flu Fraunhofer Project
* Fraun folks coming to get trained next week. RNA isolation. <br>
* Fraun folks coming to get trained next week. RNA isolation. <br>
* IBC for Fraun flu. approved? <br>
* IBC for Fraun flu. approved? <br>
<br>
<br>
† SEPSIS Project <br>
† SEPSIS Project <br>
* Revision submitted 5/20. <br>
* Revision submitted 5/20. <br>
* <br>  
* <br>  
<br>
†RNA project
†RNA project
* Jeff visiting TH <br>
* Jeff visiting TH <br>
* <br>
* <br>
<br>
† COBRA <br>  
† COBRA <br>  
* Test with sol-gel substrate w/o bacteria (26th) <br>
* Test with sol-gel substrate today(2nd June)<br>
* delayed due to difficulty adjusting membrane thickness <br>
* Evap system (JD and JZ)<br>
<br>
<br>
* Evap system (JD and JZ)<br>
** Let's get this to a point where we can publish and move on
** Let's get this to a point where we can publish and move on
paper 1: Evap with Sol Gel substrate.  <br>
paper 1: Evap with Sol Gel substrate.  <br>
paper 2: Covap with PMA, Rhodamine, PS beads?...virus...?<br>
paper 2: Covap with PMA, Rhodamine, PS beads?...virus...?<br>
<br>
<br>
† Virus concentration <br>
† Virus concentration <br>
Line 47: Line 47:
<br>
<br>
† Valve Array <br>
† Valve Array <br>
* Teddy will train Alex. JD. when he back from vacation.<br>
* Teddy will give training presentation(guide).<br>
<br>
<br>
† Fraunhofer: LOAC <br>
† Fraunhofer: LOAC <br>
Line 54: Line 54:
<br>
<br>
† Biointerfaces group <br>
† Biointerfaces group <br>
* Final draft with collaborators. <br>
* Schedule a meeting next week of all collaborators to discuss submission details. <br>
* Herringbone masks made. <br>
* found a method of double stacking SU-8 using mask-aligner for making Herringbone grooves. <br>
* investigate the stability under different Peclet numbers (ratio of hydrodynamic force and Brownian force).<br>
* investigate the stability under different Peclet numbers (ratio of hydrodynamic force and Brownian force).<br>
* asked Brett to take a photo of the device for the paper. <br>
* MC and Jason will get SEM images of PDMS mold with thin gold coating of nano-size on its surface. <br>
* draft paper2 will be started soon. <br>
<br>
<br>
† CIMIT- Colson Grant<br>
† CIMIT- Colson Grant<br>
* IRB still in revision.<br>
* IRB still in revision.<br>
*<br>
*<br>
<br>
 
† PCR <br>
† PCR <br>
* PCR 2 paper draft. <br>
* PCR 2 paper draft. <br>
* will develop large scale particle modeling in full PCR model (30 PCR cycles or 20 PCR cycles) <br>
* developed PEG-coating protocol, need do on-chip experiment. Do you need to use PEG-NH2? hoe about just PEG terminate PEG? <br>


† RCA/HDA<br>
† RCA/HDA<br>
* <br>
* Getting solution back out is still an issue. <br>
* Integrated chip worked(19th)
* Integrated chip worked (19th). Accumulating multiple runs. (Check into primer lifetime) <br>
* 3 more chips are tested when Sonali back(28th Thu). <br>
* Evaporation problems near edges. Maybe design change? <br>
* Valve characterization needed<br>
* Teflon issue with the enzyme? Check into it. <br>
<br>
 


† Silica Optimization (Lambda): <br>
† Silica Optimization (Lambda): <br>
 
* Present Bioan. Data to Jeff. <br>
*Run single PCR product from QQ or Sonali the test with the bioanalyzer<br>  
* Get lower Bioan. Kit. <br>


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2 June 2009 Lab Meeting

† Announcements

  • SIGN UP FOR NEW LAB DUTIES WITH SONALI.
  • Short small team meetings. 45 min each week?

Suggested groups... please edit. Teams
1. PCR= QQ, MCK, MM
2. Bacteria = JZ, JD
3. Flu = MM, JZ, HM, JC, BC
3. Lysis? = AG, HM?

  • MicroTAS Abstracts accepted. (4) Papers due June 30th.


† Flu R01

  • RNA extraction troubleshooting.
  • Absolute Quantification assays: TH this week.
  • FTIR

† Coulter Flu Fraunhofer Project

  • Fraun folks coming to get trained next week. RNA isolation.
  • IBC for Fraun flu. approved?


† SEPSIS Project

  • Revision submitted 5/20.

†RNA project

  • Jeff visiting TH

† COBRA

  • Test with sol-gel substrate today(2nd June)
  • delayed due to difficulty adjusting membrane thickness
  • Evap system (JD and JZ)


    • Let's get this to a point where we can publish and move on

paper 1: Evap with Sol Gel substrate.
paper 2: Covap with PMA, Rhodamine, PS beads?...virus...?

† Virus concentration



† Valve Array

  • Teddy will give training presentation(guide).


† Fraunhofer: LOAC

  • Paper revised.
  • Bonding ongoing with MIT folks


† Biointerfaces group

  • Schedule a meeting next week of all collaborators to discuss submission details.
  • found a method of double stacking SU-8 using mask-aligner for making Herringbone grooves.
  • investigate the stability under different Peclet numbers (ratio of hydrodynamic force and Brownian force).
  • asked Brett to take a photo of the device for the paper.
  • MC and Jason will get SEM images of PDMS mold with thin gold coating of nano-size on its surface.
  • draft paper2 will be started soon.


† CIMIT- Colson Grant

  • IRB still in revision.

† PCR

  • PCR 2 paper draft.
  • developed PEG-coating protocol, need do on-chip experiment. Do you need to use PEG-NH2? hoe about just PEG terminate PEG?

† RCA/HDA

  • Getting solution back out is still an issue.
  • Integrated chip worked (19th). Accumulating multiple runs. (Check into primer lifetime)
  • Evaporation problems near edges. Maybe design change?
  • Teflon issue with the enzyme? Check into it.

† Silica Optimization (Lambda):

  • Present Bioan. Data to Jeff.
  • Get lower Bioan. Kit.


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