Klapperich Lab:Notebook/Lab Meeting Notes/2009/06/02: Difference between revisions

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* Fraun folks coming to get trained next week. RNA isolation. <br>
* Fraun folks coming to get trained next week. RNA isolation. <br>
* IBC for Fraun flu. approved? <br>
* IBC for Fraun flu. approved? <br>
<br>
<br>
† SEPSIS Project <br>
† SEPSIS Project <br>
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* IRB still in revision.<br>
* IRB still in revision.<br>
*<br>
*<br>
<br>
 
† PCR <br>
† PCR <br>
* PCR 2 paper draft. <br>
* PCR 2 paper draft. <br>
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† RCA/HDA<br>
† RCA/HDA<br>
* <br>
* Getting solution back out is still an issue. <br>
* Integrated chip worked(19th)<br>
* Integrated chip worked (19th). Accumulating multiple runs. (Check into primer lifetime) <br>
* FLuidics worked but assay didnt worked<br>
* Evaporation problems near edges. Maybe design change? <br>


† Silica Optimization (Lambda): <br>
† Silica Optimization (Lambda): <br>
*Present Bioan. Data to Jeff. <br>  
* Present Bioan. Data to Jeff. <br>  
* Get lower Bioan. Kit. <br>
* Get lower Bioan. Kit. <br>


Revision as of 11:33, 2 June 2009

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2 June 2009 Lab Meeting

† Announcements

  • SIGN UP FOR NEW LAB DUTIES WITH SONALI.
  • Short small team meetings. 45 min each week?

Suggested groups... please edit. Teams
1. PCR= QQ, MCK, MM
2. Bacteria = JZ, JD
3. Flu = MM, JZ, HM, JC, BC
3. Lysis? = AG, HM?

  • Vacuum chamber needs a new gasket（done）.
  • MicroTAS Abstracts accepted. Papers due June 30th.


† Flu R01

  • RNA extraction troubleshooting.
  • Mass Spec of elution? In Chemistry.- We should approach them.
  • Bioanalyzer not working.. why? LOD - Serially dilute our samples and look to see if we can see it when we dilute the contaminants and the DNA/RNA: DID NOT SEE ANYTHING.
  • Absolute Quantification assays: TH this week.
  • FTIR-ATR look at the eluant? There are some organics in the eluant. Restrict the range. Align the laser.

† Coulter Flu Fraunhofer Project

  • Fraun folks coming to get trained next week. RNA isolation.
  • IBC for Fraun flu. approved?


† SEPSIS Project

  • Revision submitted 5/20.


†RNA project

  • Jeff visiting TH


† COBRA

  • Test with sol-gel substrate todsy(2nd June)
  • delayed due to difficulty adjusting membrane thickness
  • Evap system (JD and JZ)
    • Let's get this to a point where we can publish and move on

paper 1: Evap with Sol Gel substrate.
paper 2: Covap with PMA, Rhodamine, PS beads?...virus...?


† Virus concentration



† Valve Array

  • Teddy will give training presentation(guide).


† Fraunhofer: LOAC

  • Paper revised.
  • Bonding ongoing with MIT folks


† Biointerfaces group

  • Schedule a meeting next week of all collaborators to discuss submission details.
  • Herringbone masks made.
  • investigate the stability under different Peclet numbers (ratio of hydrodynamic force and Brownian force).


† CIMIT- Colson Grant

  • IRB still in revision.

† PCR

  • PCR 2 paper draft.
  • developed PEG-coating protocol, need do on-chip experiment.
  • will develop large scale particle modeling in full PCR model (30 PCR cycles or 20 PCR cycles)

† RCA/HDA

  • Getting solution back out is still an issue.
  • Integrated chip worked (19th). Accumulating multiple runs. (Check into primer lifetime)
  • Evaporation problems near edges. Maybe design change?

† Silica Optimization (Lambda):

  • Present Bioan. Data to Jeff.
  • Get lower Bioan. Kit.


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