Kubke Lab:/Notebook/Cranial nerve development/2011/01/28
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General Entries
--MF Kubke 00:54, 27 January 2011 (EST) Personal EntriesMalisha
1. What criteria should be included when assessing the quality of a Nissl stain?
Neurons contain Nissl substance (mainly made up of RER). Nissl substance is quite basophilic therefore will be stained with basic aninline dyes. Used the following resource to began creating a set of criteria to evaluate the Nissl stained sections of the embryos [1]
2. Why is there a series of dehydration and rehydration steps ? Why is there a "differentiation step" ?
http://physio.ucsf.edu/rubenstein/protocols/PDFs/Cobos_ISH.pdf , this protocol used by another lab group also cuts at relatively a warm temperature of -16C. From their results it is the optimal temperature for the embryo brain. --Malisha Hettiarachchi 03:11, 28 January 2011 (EST) Reuben
Fabiana
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