Kubke Lab:/Notebook/Cranial nerve development/2011/02/03: Difference between revisions
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**Loaded the <del>mould</del>block onto a metallic chuck and secured it in the metallic chuck holder. | **Loaded the <del>mould</del>block onto a metallic chuck and secured it in the metallic chuck holder. | ||
{{Note|not sure I understand - What is the difference between the mould and the old plastic mold? I assume you took the block off the mould before mounting it? --[[User:M Fabiana Kubke|MF Kubke]] 03:48, 3 February 2011 (EST)}} | {{Note|not sure I understand - What is the difference between the mould and the old plastic mold? I assume you took the block off the mould before mounting it? --[[User:M Fabiana Kubke|MF Kubke]] 03:48, 3 February 2011 (EST)}} | ||
{{Note|Can you please clarify, what is the difference between this metallic mold and the old plastic mould? What prompted this change?--[[User:M Fabiana Kubke|MF Kubke]] 22:52, 3 February 2011 (EST)}} | |||
**Built up OCT slowly to support the large block. {{Note|Adding OCT too quickly causes the frozen OCT to melt.}} | **Built up OCT slowly to support the large block. {{Note|Adding OCT too quickly causes the frozen OCT to melt.}} | ||
**Using a razor blade, by hand, I trimmed the block into a cone shape and removed enough OCT so that the block could fit between the chuck holder and the knife. | **Using a razor blade, by hand, I trimmed the block into a cone shape and removed enough OCT so that the block could fit between the chuck holder and the knife. |
Revision as of 20:52, 3 February 2011
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