Kubke Lab:Research/CND/Records2010-2011Summer/RC005: Difference between revisions
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*Allantois has <del>vesicularized</del> and is roughly the same size as the midbrain (indicative of ST20). | *Allantois has <del>vesicularized</del> and is roughly the same size as the midbrain (indicative of ST20). | ||
{{Note|what do you mean by vesicularised? --[[User:M Fabiana Kubke|MF Kubke]] 00:39, 27 January 2011 (EST)}} | {{Note|what do you mean by vesicularised? --[[User:M Fabiana Kubke|MF Kubke]] 00:39, 27 January 2011 (EST)}} | ||
(see [[Kubke Lab:/Notebook/Cranial nerve development/2011/01/10|Notebook entry 10th January 2011]]) | |||
<br>'''Fixation''': | <br>'''Fixation''': | ||
<br>'''Cryoprotection''': Was stored in PFA, staged in .9% saline and then replenished with PFA. | <br>'''Cryoprotection''': Was stored in PFA, staged in .9% saline and then replenished with PFA. | ||
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=Experiment details= | =Experiment details= | ||
<br>'''Objective''':To use as a practice embryo for staging. | <br>'''Objective''':To use as a practice embryo for staging. | ||
<br>'''Procedure''': Staged the embryo by observing the embryo under a dissection microscope and comparing its morphological properties with those described in the Hamburger and Hamilton (1951) paper whilst looking at our own compilation of images organized by stage. | <br>'''Procedure''': Staged the embryo by observing the embryo under a dissection microscope and comparing its morphological properties with those described in the Hamburger and Hamilton (1951) paper whilst looking at our own compilation of images organized by stage. | ||
Revision as of 01:36, 27 January 2011
| Cranial Nerve Development | Experiment |
Embryo details
Species: Gallus gallus domesticus
Embryo Name: RC005
Embryo stage: ST20
- (Note: That entry does not show whether I confirmed the stage of this embryo or not. Can you please clarify? --MF Kubke 00:38, 27 January 2011 (EST))
Staging description: Staged by Reuben according to the Hamburger and Hamilton (1951) staging system.
- Wing and leg buds barely formed.
- The leg is slightly longer than the wing.
- Allantois has
vesicularizedand is roughly the same size as the midbrain (indicative of ST20).
- (Note: what do you mean by vesicularised? --MF Kubke 00:39, 27 January 2011 (EST))
(see Notebook entry 10th January 2011)
Fixation:
Cryoprotection: Was stored in PFA, staged in .9% saline and then replenished with PFA.
Material label and storage:Stored in vial filled with PFA labeled RC005 and placed in a container labeled 'Reuben's staged embryos' next to the dissection microscope.
Experiment details
Objective:To use as a practice embryo for staging.
Procedure: Staged the embryo by observing the embryo under a dissection microscope and comparing its morphological properties with those described in the Hamburger and Hamilton (1951) paper whilst looking at our own compilation of images organized by stage.
Comments:
Results
Embryo is approximately ST20.
Images
Summary
This embryo may be used in further experiments as approximately a ST20 however confirmation is needed.
