Lauren M. Magee Week 2

Word Definitions

• permease-encoding
• biosynthetic
• assimilation
• extracellular
• intracellular
• flux
• biomass
• parameters
• cultured
• transferase

Paper Outline

Introduction

Ammonia is a preferred nitrogen source for Saccharomyces cerevisiae. Nitrogen metabolism is regulated at both the level of gene expression and the level of enzyme activity. Research has pointed out the influence of the concentration of ammonia and the rate of ammonia assimilation. Because of this interaction, the researchers hypothesized that the ammonia flux may have a larger impact than the concentration of ammonia in nitrogen metabolism. To study this, the researchers observed cultures having the same levels of flux but having feeds with different ammonia concentrations.

Material & Methods

S. cerevisiae was used to observe the effects of differing ammonia concentrations gene expression and enzyme activities. S. cerevisiae was continuously cultures and then feed ammonia in concentrations ranging from 29 to 118 mM along with a fixed glucose concentration and dilution rate.

Lauren M. Magee

1. Week 1
   • BIOL398-04/S15:Week 1
   • BIOL398-04/S15:Class Journal Week 1
2. Week 2
   • BIOL398-04/S15:Week 2
   • Lauren M. Magee Week 2
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3. Week 3
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   • Lauren M. Magee Week 3
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4. Week 4
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5. Week 5
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6. Week 6
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7. Week 7
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8. Week 8
   • Assignment Cancelled
9. Week 9
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   • Lauren M. Magee Week 9
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10. Week 10
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    • Lauren M. Magee Week 10
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11. Week 11
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12. Week 12
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13. Week 13
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    • Lauren M. Magee Week 13
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14. Week 14
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    • Lauren M. Magee Week 14
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15. Week 15
    • BIOL398-04/S15:Week 15
    • Lauren M. Magee Week 15
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