Magni:Back Door

Home        Contact        Lab Members        Publications        Research        Internal        Protocols        Data       

Antibiotics

• Ampicillin - 100 mg/ml in deionized water (-20°C)
• Chloramphenicol - 34 mg/ml in ethanol (-20°C)
• Kanamycin - 50 mg/ml in deionized water (-20°C)

Inducers and substrates

• HSL (3-oxo-C6-homoserine lactone) - 2 mM in water (-20°C)
• aTc (Anhydrotetracycline) - 2 mg/ml in ethanol 70% or 100 ug/ml in ethanol 70% (-20°C) LIGHT SENSITIVE
• IPTG Ready Made - 200 mM (-20°C)
• X-Gal - 40 mg/ml in DMF (-20°C)

E. coli strains

• BW20767 (Registry)

• BW23474 (CGSC)
  • pir-116 strain, used to propagate the R6K conditional replication origin at high copy

• BW25141 (CGSC)
  • pir+ strain, used to propagate the R6K conditional replication origin at medium copy

• BW25142 (CGSC)
  • pir-116 strain, used to propagate the R6K conditional replication origin at high copy (equivalent to BW23474)

• CW2553 + pJat8 (Registry)

• DB3.1 (Invitrogen)
  • can propagate plasmids with ccdB toxin

• DH5alpha (Invitrogen)
  • general purpose cloning
  • blue/white ok

• KRX (Promega)
  • overexpresses LacI via the lacIq mutation on the F plasmid
  • can be used for autoinduction protocol (see Promega handbook)
  • blue/white ok

• MC1061 (CGSC)
  • no blue/white screening

• MG1655 (CGSC)
  • K-12 sequenced strain
  • no blue/white screening

• MG1655-Z1 (Univ of Washington)
  • MG1655 with lacI- and tetR- overexpression cassettes in the genome, in the attB(lambda) locus

• TOP10 (Invitrogen)
  • general purpose cloning
  • blue/white ok

• XL1-Blue (Stratagene)

Important glycerol stocks