Magni:Protocols/Resuspension: Difference between revisions
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(New page: {{Magni}} <div style="padding: 10px; width: 720px; border: 5px solid #000000;"> = Plasmid resuspension from iGEM kit plates = *Check on the http://partsregistry.org web site the kit plate...) |
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Latest revision as of 12:04, 31 July 2012
Plasmid resuspension from iGEM kit plates
- Check on the http://partsregistry.org web site the kit plate and the exact coordinate of the desired plasmid.
- Take the kit plates, stored in the -20degC freezer. They contain dry DNA with a red dye.
- Take 15 ul of Milli-Q water (from the filter of the Millipore machine) with a P20 pipetman.
- Make a hole in the foil covering the well by using the tip and resuspend the dry DNA several times. The DNA solution should become red.
- Transfer the DNA into a 0.5-ml tube and transform 2-5 ul into competent cells.
- Store the remaining DNA at -20degC.
