McClean:Protocols: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(→Yeast) |
|||
| Line 46: | Line 46: | ||
==Stock Solutions== | ==Stock Solutions== | ||
*[[McClean: Carbenicillin Stock Solution| Carbenicillin Stock Solution]] | *[[McClean: Carbenicillin Stock Solution| Carbenicillin Stock Solution]] | ||
*[[McClean: Ladder_Stock_for_PCR | DNA Ladder Stock for PCR]] | *[[McClean: Ladder_Stock_for_PCR | DNA Ladder Stock for PCR]] | ||
*[[McClean: Bacterial Glycerol 65%| Bacterial Glycerol 65%]] | *[[McClean: Bacterial Glycerol 65%| Bacterial Glycerol 65%]] | ||
*[[McClean: PBS/0.1%Tween| PBS/0.1%Tween]] | |||
==Microfluidics== | ==Microfluidics== | ||
*[[McClean: ConA | Concanavalin A Solution for Microfluidics]] | *[[McClean: ConA | Concanavalin A Solution for Microfluidics]] | ||
Revision as of 07:20, 6 October 2011
Back to McClean Lab
Basics
Yeast
- Basic Chemostat Guide
- Bayanus Transformation
- Colony PCR (Yeast)
- Fluorescence in situ Hybridization
- Mating Type Testers
- Random Spore Prep
- Sporulation
- Tetrad Dissection
- URA Pop-out
- Yeast Recombinational Cloning
- Yeast Transformation (S. cerevisiae)
- YMC Induction in Chemostat
Bacteria
PCR
Flow Cytometry
- General Flow Cytometry Procedure
- Cycloheximide Concentration Assay
- Flourecent Protein Folding Time Assay
- Induction time course
- Phase Plane Mapping
Media
- Low Fluorescence Media
- Quickie YPD supplemented with Adenine
- Low Fluorescence Agar Membranes
- KS Amino Acid Supplement
