Mimulus:Seed Sterilization: Difference between revisions
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Line 3: | Line 3: | ||
===Supplies=== | ===Supplies=== | ||
*70% Ethanol | *70% Ethanol | ||
* | *20% Bleach | ||
*Sterile Water | *Sterile Water | ||
*Seeds | *Seeds |
Revision as of 13:12, 1 February 2007
Seed Sterilization
Supplies
- 70% Ethanol
- 20% Bleach
- Sterile Water
- Seeds
Methods
- Put seeds in an Eppendorf tube
- Add 200uL of 70% EtOH to tube
- Immediately add 600 uL of Water. Do only 2 tubes at a time, because the alcohol treatment is harsh and might affect germination if is left on seeds too long
- Immediately suck off 600 uL of supernatant
- Repeat steps 3 and 4 four times
- Set aside tubes and repeat steps 1 to 5 for all the remaining tubes
- Add 200 uL of 20% bleach to each tube
- Let tubes sit for 10 minutes
- Add 600 uL of water
- Suck off 600 uL of the supernatant
- Repeat steps 9 and 10 four or five times