NanoBio:Protocols: Difference between revisions
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*[[NanoBio: Site-Directed Mutagenesis | Site-Directed Mutagenesis]] | *[[NanoBio: Site-Directed Mutagenesis | Site-Directed Mutagenesis]] | ||
*[[NanoBio: Restriction Digest | Restriction Digest]] | *[[NanoBio: Restriction Digest | Restriction Digest]] | ||
*[[Silver: Quick & Dirty Digest & Ligation | Quick & Dirty Digest & Ligation]] | *[[Silver: Quick & Dirty Digest & Ligation | Quick & Dirty Digest & Ligation]] | ||
*[[Silver: Bacterial Transformation | Bacterial Transformation]] | *[[Silver: Bacterial Transformation | Bacterial Transformation]] | ||
Revision as of 18:51, 29 January 2008
Nano Synth Bio
General Molecular Biology
Current Protocols in Molecular Biology
Sub-cloning using Biobricks or Biofusion Vectors
- Note: Many of these are protocols that I used and/or developed as a postdoctoral fellow in Pam Silver's lab.
- Strategy for Making Parts
- Designing Parts or Genes
- Commonly Used Plasmids
- Commonly Used Primers
- PCR
- Oligonucleotide Inserts
- Overlapping Oligonucleotide Inserts
- Site-Directed Mutagenesis
- Restriction Digest
- Quick & Dirty Digest & Ligation
- Bacterial Transformation
- Plasmid Verification
- Ligation
- 3 Antibiotic Assembly
- Bacterial Transformation
- Plasmid Verification
Genomic Integration into S. cerevisiae
Lipid Bilayers
Care of Synechocystis sp 6803
Primers
- Biobricks & Biofusion Sub-cloning
- Synechocystis sp. 6803
- Shewanella oneidensis MR-1
- Guidelines on Talking or Writing about your project
- Microbial Respiration
