NanoBio: BG-11 media

BG-11 liquid media

• Cyanobacteria, including Synechocystis PCC 6803, are typically grown in BG-11 media.
• Obtain 50x BG-11 media from Sigma-Aldrich (catalog # C3061-500ML).
• In the biosafety cabinet, mix 20 mL 50x BG-11 media and 980 mL Millipore water.
• Autoclave for 15 minutes at 121C in 500 mL bottles to sterilize.

• Contents of 1x liquid media:
  • NaNO3 1.5 g/L
  • K2HPO4 0.04 g/L
  • MgSO4.7H2O 0.075 g/L
  • CaCl2.2H2O 0.036 g/L
  • Citric acid 6.0 mg/L
  • EDTA 1.0 mg/L
  • Na2CO3 0.02 g/L
  • Ferric ammonium citrate 6.0 mg/L
  • H3BO3 2.86 mg/L
  • MnCl2.4H2O 1.81 mg/L
  • ZnSO4.7H2O 0.222 mg/L
  • Na2MoO4.2H2O 0.39 mg/L
  • CuSO4.5H2O 0.079 mg/L
  • Co(NO3)2.6H2O 0.0494 mg/L
  • Distilled water 1.0 L
  • sources: Sigma-Aldrich & ATCC (media 616)

BG-11 plates

• For 1 L, or ~2 sleeves of plates, place in a 2L flask:
  • 10 mL 1 M TES pH 8.2 (Sigma catalog #)
  • 3 g sodium thiosulfate (Sigma, catalog #)
  • 15 g Bacto-agar (catalog #)
  • 20 mL 50x BG-11 media (Sigma catalog #)
  • 980 mL distilled water
  • a stirring bar

• Autoclave at 121C for 20 minutes on the liquid cycle.
• Allow to cool, with stirring, until ~50C or cool enough to handle.

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