OhioMod2013:Methods/secondary

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Secondary Functionalization of CP Nanoparticles

Now that we have DNA encapsulated within calcium phosphate nanoparticles that are well-dispersed in an aqueous solution, we now have the option of conjugating secondary functional groups on the surface of the particle, by using amide formation on the citrate-coated nanoparticles.

Phenylethylene Glycol

PEG is a non-toxic water-soluble polyether component that is useful for in vivo applications due to the ability of PEG to shield the nanoparticle from the immune system and the reticuloendothelial system. PEG-coated CP nanoparticles have seen many applications including tumour imaging[1] and systemic deliver of siRNA to tumor[2].

• Prepare aqueous solution of ethyl-3-(3-dimethylaminopropyl) carbodiimide(EDCI) and methoxyPEG-amine).
• Add to 1 mL of the post-HPLC CP nanoparticle suspension. React for 18 hr at 40 °C with continuous stirring.
• Dialzye the particles to remove excess EDCI and unreacted methoxyPEG-amine.

References

1. Altinoğlu EI, Russin TJ, Kaiser JM, Barth BM, Eklund PC, Kester M, and Adair JH. Near-infrared emitting fluorophore-doped calcium phosphate nanoparticles for in vivo imaging of human breast cancer. ACS Nano. 2008 Oct 28;2(10):2075-84. DOI:10.1021/nn800448r |
2. Li J, Chen YC, Tseng YC, Mozumdar S, and Huang L. Biodegradable calcium phosphate nanoparticle with lipid coating for systemic siRNA delivery. J Control Release. 2010 Mar 19;142(3):416-21. DOI:10.1016/j.jconrel.2009.11.008 |