Paulsson:Electroporation competent MC1061: Difference between revisions
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use 100 ul per transformation'''FINISHED''' | use 100 ul per transformation'''FINISHED''' | ||
''Batch ~03/08'', labeled "MC1061" with red ink, ~200ul aliquots<Br> | ''Batch ~03/08'', labeled "MC1061" with red ink, ~200ul aliquots<Br>'''FINISHED''' | ||
use ~95 ul per transformation; transformation efficiency ~1.6 x 10(8) cfu per ug plasmid DNA <Br> | use ~95 ul per transformation; transformation efficiency ~1.6 x 10(8) cfu per ug plasmid DNA <Br> | ||
made by Anna Andersson (using 500ml conical tubes for spins), tested by Per using pCR2.1 (1ng/ul stock) | made by Anna Andersson (using 500ml conical tubes for spins), tested by Per using pCR2.1 (1ng/ul stock) | ||
''Batch 06/25/08'', labeled "MC1061" with black ink, 105ul aliquots, made by Per<Br> | ''Batch 06/25/08'', labeled "MC1061" with black ink, 105ul aliquots, made by Per<Br>'''FINISHED''' | ||
3.4 x 10(8) ampR cfu per ug pDNA (transformation efficiency tested with 50ul cells and 1ng pCR2.1 pDNA)<br> | 3.4 x 10(8) ampR cfu per ug pDNA (transformation efficiency tested with 50ul cells and 1ng pCR2.1 pDNA)<br> | ||
Parallel batch prepared using 500ml Corning Centrifuge Tubes (rather than std bottle)<br> | Parallel batch prepared using 500ml Corning Centrifuge Tubes (rather than std bottle)<br>'''FINISHED''' | ||
labeled "MC1061" with black ink and one BLUE DOT, 105ul aliquots<Br> | labeled "MC1061" with black ink and one BLUE DOT, 105ul aliquots<Br> | ||
3.0 x 10(8) ampR cfu per ug pDNA (transformation efficiency tested with 50ul cells and 1ng pCR2.1 pDNA; ampR cfu per cfu = 1/2000) | 3.0 x 10(8) ampR cfu per ug pDNA (transformation efficiency tested with 50ul cells and 1ng pCR2.1 pDNA; ampR cfu per cfu = 1/2000) | ||
Revision as of 09:04, 18 August 2008
Batch 11/27/06, labeled with one red dot, made by Per
1.5 x 10(9) cells per ug (transformation efficiency tested with ts-Rep pDNA)
50ul aliquots, some tubes labeled "2x" contain 100ul of cells FINISHED
Batch 01/16/07, labeled with red ink, made by Per
6 x 10(9) cells per ug (transformation efficiency tested with pCR2.1 pDNA)
95ul aliquots, can use for trfs.
FINISHED
Batch 03/09/07, labeled "MC1061" with blue ink, 104ul aliquots, made by Per
1 x 10(9) cells per ug (transformation eff. tested w/ 1ng pSilver's Venus, 50ul cells)FINISHED
Batch 06/22/07, labeled "MC" with blue ink, made by Per
1.4 x 10(9) cells per ug (transformation efficiency tested with 100ul cells and 1ng pCR2.1 pDNA)
100 and 200ul aliquotsFINISHED
Batch 07/07, labeled "MC1061" with black ink, ~200ul aliquots, made by Per
use 100 ul per transformationFINISHED
Batch ~03/08, labeled "MC1061" with red ink, ~200ul aliquots
FINISHED
use ~95 ul per transformation; transformation efficiency ~1.6 x 10(8) cfu per ug plasmid DNA
made by Anna Andersson (using 500ml conical tubes for spins), tested by Per using pCR2.1 (1ng/ul stock)
Batch 06/25/08, labeled "MC1061" with black ink, 105ul aliquots, made by Per
FINISHED
3.4 x 10(8) ampR cfu per ug pDNA (transformation efficiency tested with 50ul cells and 1ng pCR2.1 pDNA)
Parallel batch prepared using 500ml Corning Centrifuge Tubes (rather than std bottle)
FINISHED
labeled "MC1061" with black ink and one BLUE DOT, 105ul aliquots
3.0 x 10(8) ampR cfu per ug pDNA (transformation efficiency tested with 50ul cells and 1ng pCR2.1 pDNA; ampR cfu per cfu = 1/2000)
Batch 08/15/08, labeled "MC1061" with blue ink, 105ul aliquots (37x from 2L OD 0.5), made by Per
Prepared using 500ml Corning Centrifuge Tubes
6.0 x 10(8) ampR cfu per ug pDNA (transformation efficiency tested with 50ul cells and 1ng pCR2.1 pDNA)
