Paulsson:Nanodrop: Difference between revisions
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===Getting started=== | ===Getting started=== | ||
===Measuring S. pombe cultures=== | |||
===Measuring E. coli cultures=== | ===Measuring E. coli cultures=== | ||
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each data point is the average value of 3 samples, measured in triplicate | each data point is the average value of 3 samples, measured in triplicate | ||
[[Image:nanodrop061214.jpg]] | [[Image:nanodrop061214.jpg]] | ||
Revision as of 14:23, 15 December 2006
Nanodrop Spectrophotometer
Location
Getting started
Measuring S. pombe cultures
Measuring E. coli cultures
It seems that using more than the minimal 2ul is important for obtaining reliable measurements. 4ul will do.
- OD600 = 0.1 is equivalent to approximately 4 x 10(8) cells/ml
determined at one dilution with MC1061 at mid-log, 12/11/06, Per M.
- OD600 measurement linear is useful range
each data point is the average value of 3 samples, measured in triplicate File:Nanodrop061214.jpg
