Paulsson:Nanodrop

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Nanodrop Spectrophotometer

Measuring E. coli cultures

It seems that using more than the minimal 2ul can be important for obtaining reliable measurements. 3ul works consistently.

• OD600 = 0.1 is equivalent to approximately 5 x 10(8) cells/ml

Determined at one dilution with MC1061 by counting cells with hemacytometer: OD600 of 0.1 = 4 x 10(8) cells/ml (12/11/06, Per M.)
Determined at one dilution with XL1-BLue by plating cells: OD600 of 0.1 = 5.9 x 10(8) cells/ml (12/19/06, Per M.)

• OD600 measurement linear between values of 0.005 and 0.35