Prince:Mass Spectrometry: Difference between revisions
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===UPLC=== | ===UPLC=== | ||
*Troubleshooting the Eksigent UPLC system | |||
* [[Prince:UPLC|UPLC]] (Fittings/tubing/flow rates) | * [[Prince:UPLC|UPLC]] (Fittings/tubing/flow rates) | ||
** 10 micron needles provide best nano-spray at 200-300 nl/min | ** 10 micron needles provide best nano-spray at 200-300 nl/min |
Revision as of 13:29, 31 December 2010
New Users
We recommend you peruse this site and become more familiar with the content. Additionally, you may visit this site specifically, where we have compiled information pertinent into an introduction for you.
Introduction to Mass Spectrometry
Sample Preparation
Proven Methods:
- FASP (filter aided sample preparation)
- In Gel FASP
Side Notes:
Equipment
Gases
[From the wall out]
- Helium UHP [LTQ] (40psi)
- Nitrogen UHP [HCD] (100psi)
- Nitrogen 99% (this is the normal nitrogen in the stock room) [sheath & Orbitrap bath] (100psi)
- Nitrogen? Normal? psi? [for UPLC]
UPLC
- Troubleshooting the Eksigent UPLC system
- UPLC (Fittings/tubing/flow rates)
- 10 micron needles provide best nano-spray at 200-300 nl/min
Tuning/Calibration
Mascot Restart
user@jp1:~$ ls /etc/init.d/mascot /etc/init.d/mascot user@jp1:~$ sudo /etc/init.d/mascot status [sudo] password for user: user@jp1:~$ sudo /etc/init.d/mascot restart * Mascot monitor is not running * Mascot monitor started user@jp1:~$