Reagents for SPR: Difference between revisions
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|EDC stock solution 0.4M<br> | |EDC stock solution 0.4M<br> | ||
resuspend fresh ultrapure reagent in milli-Q water<br> | resuspend fresh ultrapure reagent in milli-Q water<br> | ||
aliquote into | aliquote into 200μL portions directly into vials to be used on the instrument<br> | ||
freeze the aliquotes and store at -20 for no more then 2 months | freeze the aliquotes and store at -20 for no more then 2 months | ||
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Revision as of 14:02, 19 May 2008
| reagents | purpose | Mr | handling instructions |
| 1-ethyl-3-(3-dimethyl
aminopropyl) carbodiimide (EDC) |
amine coupling | 191.70 | EDC stock solution 0.4M resuspend fresh ultrapure reagent in milli-Q water |
| N-hydroxysuccinimide (NHS) |
amine coupling | 115.09 | NHS stock solution 0.1M prepare and store as desribed for EDC |
