RiPS
From OpenWetWare
Ribosomes Per Second
A standard unit to measure translational activity of an mRNA molecule.
RiPS per mRNA are equivalent to the rate of the limiting step in ribosome initiation and elongation.
| Study | Value | Note |
|---|---|---|
| Kennell et al. [1] | 0.3 Proteins mRNA-1 s-1 | Measured for the lac operon in E. coli |
| De Smit et al. [2] | 0.5 Proteins mRNA-1 s-1 | Assumed this value based on (insert paper here) |
| Braff & Conboy | 0.039-0.55 Proteins mRNA-1 s-1 | Measured using BBa_I7107 and BBa_I7109 on pSB3K3 and pSB4A3. |
References
- Kennell D and Riezman H. Transcription and translation initiation frequencies of the Escherichia coli lac operon. J Mol Biol. 1977 Jul;114(1):1-21. DOI:10.1016/0022-2836(77)90279-0 |
- de Smit MH and van Duin J. Translational standby sites: how ribosomes may deal with the rapid folding kinetics of mRNA. J Mol Biol. 2003 Aug 22;331(4):737-43. DOI:10.1016/s0022-2836(03)00809-x |
