SBB12Ntbk-DanielChao: Difference between revisions
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Using protocol as described here: [[Template:SBB-Protocols_PCR1 | Wobble Reaction]] | Using protocol as described here: [[Template:SBB-Protocols_PCR1 | Wobble Reaction]] | ||
==[[User:Daniel Chao|Daniel]] 14:00, 17 February 2012 (PST)== | |||
Performed short-fragment Zymo on wobble product, using protocol as described here [[Template:SBB-Protocols_Zymo2 | Small-Frag Zymo Cleanup]]<br> | |||
==[[User:Daniel Chao|Daniel]] 12:30, 21 February 2012 (PST)== | |||
Ran gel of two PCR products used for SOEing [[SBB12_gels | Gel pics]] <br> | |||
Cut out gel and purified using [[Template:SBB-Protocols_Zymo3 | Zymo Gel Purification]]<br> | |||
Performed digest reaction on Zymo wobble product (1 hour in 37C), then purified using Small-Frag Zymo Cleanup. | |||
Revision as of 13:36, 21 February 2012
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Daniel 11:30, 16 February 2012 (PST)
Prepared PCR reactions for sb1221:
PCR ca998/gfRevPR on pBjk2741-jtk2801 (160bp, A)
Placed in 100-500 PCR tray
PCR dc5002/g00101 on pBgl00001-Brp0006 (1100bp, B)
Placed in 1K-2K PCR tray
Using protocol as described here: Cloning by PCR
Prepared Wobble reaction for sbb1228
Wobble dc5003/dc5004 (64bp, wobpdt)
Using protocol as described here: Wobble Reaction
Daniel 14:00, 17 February 2012 (PST)
Performed short-fragment Zymo on wobble product, using protocol as described here Small-Frag Zymo Cleanup
Daniel 12:30, 21 February 2012 (PST)
Ran gel of two PCR products used for SOEing Gel pics
Cut out gel and purified using Zymo Gel Purification
Performed digest reaction on Zymo wobble product (1 hour in 37C), then purified using Small-Frag Zymo Cleanup.
