SEED/2011/Schedule: Difference between revisions

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(New page: <div class=tabs> <ul> <li>Home</li> <li id="current">Schedule</li> <li>Links</li> </ul> </div> <div class="tabcontent"> <div style=...)
 
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| [[../Day 1/]]
| [[../Day 1/]]
| Feb. 5
| Feb. 5
| safety training, [[Media:7.02-training-lab.pdf|training lab]]
|  
*Introductions
*Molecular Biology Review
*Units Review
|  
|  
* Homework #1: [[Media:SEED_HW1-2011.doc|doc]] [[Media:SEED_HW1-2011.pdf|pdf]]
* Homework #1: [[Media:SEED_HW1-2011.doc|doc]] [[Media:SEED_HW1-2011.pdf|pdf]]
** read [[Media:AdventuresComic.pdf|pdf]]
** read [[Media:AdventuresComic.pdf|comic]]
** iGEM Questions
** iGEM Questions
** Ideas (3-5) for what you want to engineer biology to do
** Ideas (3-5) for what you want to engineer biology to do
** Training lab questions
** Training lab questions
** Data Analysis
** Data Analysis
** Pre-Lab
| Lab Day #1: Serial Dilutions [[Media:SEED_pipette.pdf|pdf]]  [[Media:SEED_pipette.doc|doc]]  
| Lab Day #1: Serial Dilutions [[Media:SEED_pipette.pdf|pdf]]  [[Media:SEED_pipette.doc|doc]]  
|-
|-

Revision as of 17:30, 13 February 2011

[[../Overview/]] of the main lab project

Class Date Agenda Homework Lab Protocol
[[../Day 1/]] Feb. 5
  • Introductions
  • Molecular Biology Review
  • Units Review
  • Homework #1: doc pdf
    • read comic
    • iGEM Questions
    • Ideas (3-5) for what you want to engineer biology to do
    • Training lab questions
    • Data Analysis
Lab Day #1: Serial Dilutions pdf doc
[[../Day 2/]] Feb. 12 SynBio Overview, sterile technique, colony streaking, antibiotic resistance
  • Homework #2:
Lab Day #2: Plate Streaking pdf doc
[[../Day 3/]] Feb. 26 miniprep, digest
  • Homework #3:
  • Pre-Lab #3:
  • Lab Day #3: Plasmid Mini-Prep pdf doc
  • Restriction Digest pdf doc
[[../Day 4/]] Mar. 5 PCR and gel
  • Homework #4:
[[../Day 5/]] Mar. 12 gel purification:
  • Homework #5:
Lab Day #5: Gel Purification pdf doc
[[../Day 6/]] Mar. 26 ligation, transformation
  • Pre-Lab #6:
Lab Day #6: Ligation pdf doc
[[../Day 7/]] Apr. 2 prep for sequencing, glycerol

final project notes:

  • Homework #7:
  • Pre-Lab #5:
Lab Day #7: Transformation pdf doc
[[../Day 8/]] Apr. 9 β-gal assays, growth curves, viable cell titer, analyze sequencing
  • Homework #8:
Lab Day #8: Beta-Galactosidase: Part I pdf doc
[[../Day 9/]] Apr. 23 device characterization and composability
  • Homework #9: TBA
Lab Day #9: Beta-Galactosidase: Part II pdf doc
[[../Day 10/]] April 30 data analysis, finish projects
  • Final: TBA
Final Lab: pdf