Sentelab:Notebook/gene synthesis/2010/09/18: Difference between revisions
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Burak Yilmaz (talk | contribs) (Autocreate 2010/09/18 Entry for Sentelab:Notebook/gene_synthesis) |
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== | ==1st PCR product purification by PCR purification kit = | ||
#5 volumes of buffer PB added to volume of the PCR reaction and mixed | |||
#A QIAquick column is plaed ina provided 2 ml collection tube | |||
#To bind DNA, the sample is applied to the QIAquick column and centrifuged for 45 sec. Flow trough is discarded and QIAquick column is placed back into the same tube. | |||
#To wash 0,75 ml buffer PE is added to the QIAquick column and centrifuged for 45 sec .Flow through is discaded and QIAquick column is placed back into the same tube. | |||
#The column is centrifuged in a 2ml collection tube for 1min | |||
#Each QIA quick column is placed in a clean 1.5 ml microcentrifuge tube. | |||
#The elute DNA for increased DNA concentration 30 ul EB buffer added to the center of the QIAquick membrane let the column stand for 1 min and than centrifuged. | |||
*Nano drop results | |||
#1-11 - gfp oligos : 100,3 ng/uL | |||
#12-22 gfp oligos : 89,7 ng/uL | |||
Revision as of 07:27, 17 September 2010
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=1st PCR product purification by PCR purification kit
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