Small molecule binding by SPR: Difference between revisions
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Drug-like small organic molecules often do not dissolve well in water. | Drug-like small organic molecules often do not dissolve well in water. Small concentration (<10%) of DMSO is used then to improve drug solubility for SPR analysis. | ||
A common technique is to add DMSO solution of drug to the aqueous buffer <cite>karlsson1</cite>. | A common technique is to add DMSO solution of drug to the aqueous buffer <cite>karlsson1</cite>. | ||
Latest revision as of 15:58, 7 April 2008
Drug-like small organic molecules often do not dissolve well in water. Small concentration (<10%) of DMSO is used then to improve drug solubility for SPR analysis. A common technique is to add DMSO solution of drug to the aqueous buffer [1].
Dielectric properties of DMSO and water are significantly different. As a result, even when the test solution contains no analyte there can be large offset in RU between the reference and test cells. Karlsson et al [1] have suggested a calibration protocol that eliminates this offset.
<biblio>
- karlsson1 pmid=10821711
<biblio>
