Smolke:Protocols
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Note: If possible, please follow the
standard
protocol format.
Contents
1
Protocols
1.1
General
1.2
Yeast
1.3
DNA
1.4
Flow Cytometer
1.5
Miscellaneous
1.6
Mammalian
1.7
Bacteria
2
Solutions
Protocols
General
How long does my reaction take?
Smolke:Plasmid prep with Epoch columns and homemade buffers
Smolke:PCR amplifying inserts from plasmids
When to use phenol-chloroform vs. columns
Smolke:Gel extraction
Smolke:Ligation
Smolke:Transformation
Smolke:Colony PCR
Smolke:Screening by digestion
Smolke:Sequencing
Yeast
Protocols
Pouring yeast dropout plates
Colony PCR (Travis' version)
Anti-V5 Western
Library-scale yeast transformations
Observations and Discussion
CSY3
DNA
Protocols
Oligos
Flow Cytometer
Protocols
Compensation
Miscellaneous
Protocols
Making coffee
Lab drinks
Dishwasher
Glass Beads
Mammalian
Bacteria
Solutions
Recipes
Pfu Buffer
Taq Buffer
10x DO Media
dNTP Mixture
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