TFB I

DH5α Competent Cells

TFB I

Reagent	F.W.	200 ml	400 ml	800 ml
30 mM Potassium acetate	98.14	0.59 g	1.18 g	2.36 g
100mM RbCl	120.9	2.42 g	4.84 g	9.68 g
10 mM CaCl2	147	0.294 g	0.59 g	1.18 g
50 mM MnCl2	197.9	1.98 g	3.96 g	7.92 g
15% Glycerol	N/A	30 mL	60 mL	120 mL

pH 5.8 w/ Acetic Acid (do not overshoot—correcting with KOH will cause irreversible precipitation). Filter sterilize.