TFB I
From OpenWetWare
Jump to navigationJump to search
Reagent | F.W. | 200 ml | 400 ml | 800 ml |
---|---|---|---|---|
30 mM Potassium acetate | 98.14 | 0.59 g | 1.18 g | 2.36 g |
100mM RbCl | 120.9 | 2.42 g | 4.84 g | 9.68 g |
10 mM CaCl2 | 147 | 0.294 g | 0.59 g | 1.18 g |
50 mM MnCl2 | 197.9 | 1.98 g | 3.96 g | 7.92 g |
15% Glycerol | N/A | 30 mL | 60 mL | 120 mL |
pH 5.8 w/ Acetic Acid (do not overshoot—correcting with KOH will cause irreversible precipitation). Filter sterilize.