TFB I

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DH5α Competent Cells

TFB I
Reagent F.W. 200 ml 400 ml 800 ml
30 mM Potassium acetate 98.140.59 g1.18 g2.36 g
100mM RbCl 120.92.42 g4.84 g9.68 g
10 mM CaCl2 1470.294 g0.59 g1.18 g
50 mM MnCl2 197.91.98 g3.96 g7.92 g
15% Glycerol N/A30 mL60 mL120 mL

pH 5.8 w/ Acetic Acid (do not overshoot—correcting with KOH will cause irreversible precipitation). Filter sterilize.

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