Talk:20.109(F07):Start-up genome engineering: Difference between revisions
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| TRpink_top | | TRpink_top | ||
| NO | | NO | ||
| | | 5’ GAT CTC GAC GGC GCC 3’ | ||
| | | | ||
| | | Sal I | ||
|- | |- | ||
| TRpink_btm | | TRpink_btm | ||
| NO | | NO | ||
| | | 5’ G ATC GGC GCC GTC GA 3’ | ||
| | | | ||
|Sfo I | |||
|- | |- | ||
| TRyellow_top | | TRyellow_top |
Revision as of 13:31, 11 September 2007
oligo name | oligo number | oligo sequence | primer record | enzymes added with insert | |
---|---|---|---|---|---|
TeacherOligo_top | NO196 | 5' GAT CTG CGC ACG CGT | teacher sample oligo record | FspI and MluI | |
TeacherOligo_btm | NO197 | 5'G,ATC,ACG,CGT,GCG,CA | |||
M13KO7seq_2101_fwd | NO198 | 5'- CAA GGC ACT GAC CCC GTT AAA ACT TAT TAC -3' | |||
M13KO7seq_2151_fwd | NO199 | 5'- AAA AGC CAT GTA TGA CGC TTA CTG GAA CGG -3' |
T/R lab
oligo name | oligo number | oligo sequence | primer record | enzymes added with insert |
---|---|---|---|---|
TRpink_top | NO | 5’ GAT CTC GAC GGC GCC 3’ | Sal I | |
TRpink_btm | NO | 5’ G ATC GGC GCC GTC GA 3’ | Sfo I | |
TRyellow_top | NO | |||
TRyellow_btm | NO | |||
TRblue_top | NO | |||
TRblue_btm | NO | |||
TRpurple_top | NO | |||
TRpurple_btm | NO | |||
TRred_top | NO | |||
TRred_btm | NO | |||
TRgreen_top | NO | |||
TRgreen_btm | NO |
W/F lab
oligo name | oligo number | oligo sequence | primer record | enzymes added with insert |
---|---|---|---|---|
WFpink_top | NO | |||
WFpink_btm | NO | |||
WFyellow_top | NO | |||
WFyellow_btm | NO | |||
WFblue_top | NO | |||
WFblue_btm | NO | |||
WFpurple_top | NO | |||
WFpurple_btm | NO | |||
WFred_top | NO | |||
WFred_btm | NO | |||
WFgreen_top | NO | |||
WFgreen_btm | NO |