Talk:20.109(S07):Start-up genome engineering
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Insert designs
T/R lab
| protein to modify | oligo name | oligo number | oligo sequence | primer record | enzyme for verification of insert |
|---|---|---|---|---|---|
| p8 | TRpink_top | NO | TCT GAA CAG AAA CTG ATA TCT GAA GAA GAC CTG GAt gca | pink oligo record | AlwN1
|
| p8 | TRpink'_btm | NO | CCTAGGTCTTCTTCAGATATCAGTTTCTGTTCAGAtgca | This restriction enzyme cuts our oligo once and the M13 twice. | |
| p3 | TRyellow'_top | NO. 28488673 | gat cTG GAA CAG AAA CTG ATA TCT GAA GAA GAC CTA | yellow oligo record | EcoRV |
| p3 | TRyellow'_btm | NO. 28488674 | gatcTAGGTCTTCTTCAGATATCAGTTTCTGTTCCA | ||
| p3 | TRblue_top | NO | gat cAG CTG GAA CAG AAA CTG ATC TCT GAA GAA GAC CTA | blue oligo record | Zra1 |
| p3 | TRblue'_btm | NO | gatcTAGGTCTTCTTCAGAGATCAGTTTCTGTTCCAGCT | ||
| p3 | TRpurple_top | NO | gat cTA GAA CAG AAA CTG ATC TCT GAA GAA GAC CTA | purple oligo record | |
| p3 | TRpurple'_btm | NO | gatcTAGGTCTTCTTCAGAGATCAGTTTCTGTTCTA | ||
| p3 | TRred_top | NO | gat cTA GAA CAG AAA CTG ATC TCT GAA GAA GAC CTA | red oligo record | XbaI and HindIII |
| p3 | TRred'_btm | NO | gatcTAGGTCTTCTTCAGAGATCAGTTTCTGTTCTA | ||
| p3 | TRgreen_top | NO | gat cTG GAA CAG AAA CTG ATC TCT GAA GAA GAC CTG CT | green oligo record | BbsI |
| p3 | TRgreen'_btm | NO | gatcAGCAGGTCTTCTTCAGAGATCAGTTTCTGTTCCA |
W/F lab
| protein to modify | oligo name | oligo number | oligo sequence | primer record | enzyme for verification of insert |
|---|---|---|---|---|---|
| p3 | WFpink_top | NO | gat c aa GAA CAG AAA CTG ATa TCT GAA GAA GAC CTa | pink primer record | |
| p3 | WFpink_btm | NO | gatctAGGGTCTTCTTCAGAtATCAGTTTCTGTTCtt | ||
| p3 | WFyellow_top | NO | gat cTA GAA CAG AAG CTT ATC TCT GAA GAA GAC CTC | yellow primer record | |
| p3 | WFyellow_btm | NO | g atc GAG GTC TTC TTC AGA GAT AAG CTT CTG TTC TA | ||
| p3 | WFblue_top | NO | gat cTC GAA CAG AAA CTG ATA TCT GAA GAA GAC CTT | blue primer record | EcoRV |
| p3 | WFblue_btm | NO | gatcAAGGTCTTCTTCAGATATCAGTTTCTGTTCGA | and pdf version | EcoRV |
| p8 | WFpurple_top | NO | TCT GAA CAG AAA CTG ATC TCT GAA GAA GAC CTG GGt gca | purple primer record | Bbs1 |
| p8 | WFpurple_btm | NO | CCCAGGTCTTCTTCAGAGATCAGTTTCTGTTCAGAtgca | ||
| p8 | WFred_top | NO | CTG GAA CAG AAA CTG ATC TCT GAA GAA GAC CTG ggt_gca | red primer record | BbsI |
| p8 | WFred_btm | NO | ccCAG GTC TTC TTC AGA GAT CAG TTT CTG TTC GAC tgca | ||
| p8 | WFgreen_top | NO | TCT GAA CAG AAA CTG ATA TCT GAA GAA GAC CTG GAt gca | green primer record | EcoRV |
| p8 | WFgreen_btm | NO | CTCAGGTCTTCTTCAGATATCAGTTTCTGTTCAGAtgca |
