Talk:20.109(S08):Start-up protein engineering (Day1): Difference between revisions
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===All class mutations=== | ===All class mutations=== | ||
<code> | <code> | ||
1 99 | 1 99 | ||
TDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIYFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGY | TDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIYFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGY | ||
P D H E P H D D | P D H E P H D D | ||
R R I K D A K P | R R I K D A K P | ||
| Line 106: | Line 106: | ||
100 148 | 100 148 | ||
ISAAQLRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK | ISAAQLRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK | ||
L R | L R | ||
T W | T W | ||
Revision as of 19:26, 13 March 2008
TR lab
| Team colour | Mutant 1 | Primer 1 (forward) | Mutant 2 | Primer 2 (forward) |
| Red | D133R | GGGAAGCAGATATCGATGGTCGTGGCCAAGTAAACTATGAAG | D133W | GGGAAGCAGATATCGATGGTTGGGGCCAAGTAAACTATGAAGAG |
| Purple | Y64P | GCTGATGGCAATGGAACGATTCCCTTTCCTGAATTTCTTACTATG | Y64K | GCTGATGGCAATGGAACGATTAAGTTTCCTGAATTTCTTACTATG |
| Blue | G98D | GACAAGGATGGGAACGACTACATCAGCGCTGCTCAGTTACGTC | G98P | GACAAGGATGGGAACCCCTACATCAGCGCTGCTCAGTTACGTC |
| Pink | G96D | CGTGTTTTTGACAAGGATGACAACGGCTACATCAGCGCTGC | G96K | GTTTTTGACAAGGATAAGAACGGCTACATCAGCGCTGCTCAG |
| Yellow | T28D | CGACAAGGATGGGGACGGCACCATCGACACAAAGGAAC | T28R | GATGGGGACGGCACCATCAGGACAAAGGAACTTGGCACCG |
| Green | Y64D | GGCAATGGAACGATTGACTTTCCTGAATTTCTGACTATGATGGC | Y64H | GGCAATGGAACGATTCACTTTCCTGAATTTCTCACTATGATGGC |
WF lab
| Team colour | Mutant 1 | Primer 1 (forward) | Mutant 2 | Primer 2 (forward |
| PINK | D56->P | CTTCTCATTATTCCCCAAGGATGGGGACGGCACCATCACC | D56->R | CTTCTCATTATTCCGCAAGGATGGGGACGGCACCATCACC |
| PURPLE | S101->L | GATGGGAACGGCTACATCCTCGCTGCTCAG | S101->T | GATGGGAACGGCTACATCACCGCTGCTCAG |
| BLUE | N60->E | GATGCTGATGGCGAAGGAACGATTTAC | N60->K | GATGCTGATGGCAAGGGAACGATTTAC |
| RED | G98->P | GTTTTTGACAAGGATGGGAACCCCTACATCAGCGCTGCTCAG | G98->W | GTTTTTGACAAGGATGGGAACTGGTACATCAGCGCTGCTCAG |
| Yellow | D56H | 5' GAT ATG ATC AAT GAA GTC CAT GCT GAT GGC AAT GGA ACG 3' | D56I | 5' GAT ATG ATC AAT GAA GTC ATT GCT GAT GGC AAT GGA ACG 3' |
| GREEN | K94->H | gcattccgtgtttttgacCACgatgggaacggctacatcagcgc | K94->A | gcattccgtgtttttgacGCGgatgggaacggctacatcagcgc |
All class mutations
1 99
TDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIYFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGY
P D H E P H D D
R R I K D A K P
K P
H W
100 148
ISAAQLRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK
L R
T W
