Talk:20.109(S09):Induce protein and evaluate DNA (Day5)
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TR O/N cultures and Gels
The table below lists the OD600 values of 1:10 dilutions of the cultures grown overnight, as well as the pellet observations.
Group | OD WT | OD Mut1 | OD Mut2 | Pellets yellow? |
---|---|---|---|---|
Note: 1% gels were run for 45 min, 1.2% gels for 60 min, both at 100V.
There seems to have been a contamination or other strange issue with the S101L DNA and/or FspI enzyme. For your final reports, please use my old data, which will be posted below later tonight.
WF O/N cultures and Gels
The table below lists the OD600 values of 1:10 dilutions of the cultures grown overnight, as well as the pellet observations.
Group | OD WT | OD Mut1 | OD Mut2 | Pellets yellow? |
---|---|---|---|---|
pics here