Talk:20.109(S11):Purify RNA and run affinity column (Day4): Difference between revisions
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|4 | |4 | ||
|24 | |24 | ||
| | |Repeat from last year's experiment | ||
|- | |- | ||
|2 | |2 | ||
|Orange | |Orange | ||
| | |2 | ||
| | |2 | ||
| | |48 | ||
| | |New experiment: Testing extremes of washing. | ||
|- | |- | ||
|3 | |3 | ||
|Yellow | |Yellow | ||
| | |50 | ||
| | |8 | ||
| | |16 | ||
| | |Last year's experimenters observed a decrease in yield after more washes. Repeating to see if the break from the general trend is due to the conditions selected or experimenter variation. | ||
|- | |- | ||
|4 | |4 | ||
|Green | |Green | ||
| | |.25 | ||
| | |4 | ||
| | |24 | ||
| | |New experiment, .25% pre-selection 8-12 to test lower limits of 8-12 recovery. | ||
|- | |- | ||
|5 | |5 | ||
|Blue | |Blue | ||
| | |10 | ||
| | |2 | ||
| | |34 | ||
| | |New experiment. Using 10% (we would have chosen 2%, but we suspect there could be contamination in our sample) but with extremes of washing. | ||
|- | |- | ||
|6 | |6 | ||
|Pink | |Pink | ||
| | |10 | ||
| | |8 | ||
| | |16 | ||
| | |Repeat from last year's | ||
|- | |- | ||
|7 | |7 |
Latest revision as of 08:10, 15 February 2011
Sign-up for column protocols
T/R
You and your partner should propose a protocol in the table below.
If you make changes to the 8-12 % due to not having enough RNA on Day 4, please note them in the final column.
Protocol# | Group | 8-12 % | Wash # (partner 1) | Wash # (partner 2) | Note changes to plan here |
---|---|---|---|---|---|
0 | Sample | 5 | 5 | 15 | Didn't have enough 6-5 when isolated RNA, so switched to 10% 8-12. |
1 | Red | .5 | 4 | 24 | Starting with a lower 8-12 concentration. |
2 | Orange | 50 | 8 | 16 | Repeat high-percentage run because the decrease in enrichment with increasing number of washes intrigued us |
3 | Yellow | 1 | 4 | 24 | starting with a lower 8-12 concentration |
4 | Green | 50 | 4 | 24 | Repeating high-percentage run for statistical reliability. |
5 | Blue | 2 | 4 | 32 | Check to see at what limit does the trend of more washes contribute to the yield hold true. |
6 | Pink | 2 | 4 | 24 | Repeat from last year's experiment |
7 | Purple | 10 | 8 | 32 | Interested in seeing the effect of introducing greater number of washes |
W/F
You and your partner should propose a protocol in the table below.
If you make changes to the 8-12 % due to not having enough RNA on Day 4, please note them in the final column.
Protocol# | Group | 8-12 % | Wash # (partner 1) | Wash # (partner 2) | Note changes to plan here |
---|---|---|---|---|---|
0 | Sample | 5 | 5 | 15 | Didn't have enough 6-5 when isolated RNA, so switched to 10% 8-12. |
1 | Red | 2 | 4 | 24 | Repeat from last year's experiment |
2 | Orange | 2 | 2 | 48 | New experiment: Testing extremes of washing. |
3 | Yellow | 50 | 8 | 16 | Last year's experimenters observed a decrease in yield after more washes. Repeating to see if the break from the general trend is due to the conditions selected or experimenter variation. |
4 | Green | .25 | 4 | 24 | New experiment, .25% pre-selection 8-12 to test lower limits of 8-12 recovery. |
5 | Blue | 10 | 2 | 34 | New experiment. Using 10% (we would have chosen 2%, but we suspect there could be contamination in our sample) but with extremes of washing. |
6 | Pink | 10 | 8 | 16 | Repeat from last year's |
7 | Purple | 50 | 4 | 24 | repeating last year's experiment that did not actually have 50% |