Tk:protein tags: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
Line 29: | Line 29: | ||
* protein solubility | * protein solubility | ||
** maltose binding protein | ** maltose binding protein | ||
** | ** Thioredoxin | ||
* Protein cleavage sites | |||
** Enterokinase | |||
*** Asp-Asp-Asp-Asp-Lys↓ | |||
*** some side cutting at basic residues | |||
** Factor Xa | |||
*** Ile-Glu/Asp-Gly-Arg↓ | |||
** Thrombin | |||
*** Leu-Val-Pro-Arg↓Gly-Ser | |||
** TEV protease | |||
*** Glu-Asn-Leu-Tyr-Phe-Gln↓Gly | |||
** Pre-scission | |||
*** Leu-Glu-Val-Leu-Phe-Gln↓Gly-Pro | |||
** |
Revision as of 11:01, 18 July 2008
- References
- PMID 12238768
- Current Protcols in Protein Science, section 9.9
- protein purification tags, antibody epitopes
- FLAG
- DYKDDDDK, pmid 8770418
- FLAG Epitope description
- c-MYC
- EQKLISEEDL
- HA
- YPYDVPDYA
- 6HIS
- HHHHHH
- Strep
- AWRHPQFGG
- Ala-Trp-Arg-His-Pro-Gln-Phe-Gly-Gly
- GST (n-terminal)
- Chitin binding
- BCCP biotin carboxyl carrier protein
- VSVG
- Thrombin cleavage domain
- LVPRGS (Leu-Val-Pro-Arg-Gly-Ser), factor Xa cleaved
- Calmodulin tag
- S-tag
- GFP protein tags
- FLAG
- protein solubility
- maltose binding protein
- Thioredoxin
- Protein cleavage sites
- Enterokinase
- Asp-Asp-Asp-Asp-Lys↓
- some side cutting at basic residues
- Factor Xa
- Ile-Glu/Asp-Gly-Arg↓
- Thrombin
- Leu-Val-Pro-Arg↓Gly-Ser
- TEV protease
- Glu-Asn-Leu-Tyr-Phe-Gln↓Gly
- Pre-scission
- Leu-Glu-Val-Leu-Phe-Gln↓Gly-Pro
- Enterokinase