Tk:protein tags
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- References
- PMID 12238768
- Current Protcols in Protein Science, section 9.9
- protein purification tags, antibody epitopes
- FLAG
- DYKDDDDK, pmid 8770418
- FLAG Epitope description
- c-MYC
- EQKLISEEDL
- HA
- YPYDVPDYA
- 6HIS
- HHHHHH
- Strep
- AWRHPQFGG
- Ala-Trp-Arg-His-Pro-Gln-Phe-Gly-Gly
- GST (n-terminal)
- Chitin binding
- BCCP biotin carboxyl carrier protein
- VSVG
- Thrombin cleavage domain
- LVPRGS (Leu-Val-Pro-Arg-Gly-Ser), factor Xa cleaved
- Calmodulin tag
- S-tag
- GFP protein tags
- T7 epitope
- MASMTGGQQMG (N terminal or internal)
- Novagen T7 tag purification kit (Novagen), pGEMEX vectors (Promega)
- Makrides 1996
- V5 tag
- GKPIPNPLLGLDST (C-terminal)
- pET, pBAD and Gateway (Invitrogen)
- McLean 2001
- FLAG
- protein solubility
- maltose binding protein
- Thioredoxin
- Protein cleavage sites [1]
- Enterokinase
- Asp-Asp-Asp-Asp-Lys↓
- some side cutting at basic residues
- Factor Xa
- Ile-Glu/Asp-Gly-Arg↓
- Thrombin
- Leu-Val-Pro-Arg↓Gly-Ser
- TEV protease
- Glu-Asn-Leu-Tyr-Phe-Gln↓Gly
- PreScission
- Leu-Glu-Val-Leu-Phe-Gln↓Gly-Pro
- Enterokinase