Tk:protein tags

• References
  • PMID 12238768
  • Current Protcols in Protein Science, section 9.9

• protein purification tags, antibody epitopes
  • FLAG
    • DYKDDDDK, pmid 8770418
    • FLAG Epitope description
  • c-MYC
    • EQKLISEEDL
  • HA
    • YPYDVPDYA
  • 6HIS
    • HHHHHH
  • Strep
    • AWRHPQFGG
    • Ala-Trp-Arg-His-Pro-Gln-Phe-Gly-Gly
  • GST (n-terminal)
  • Chitin binding
  • BCCP biotin carboxyl carrier protein
  • VSVG
  • Thrombin cleavage domain
    • LVPRGS (Leu-Val-Pro-Arg-Gly-Ser), factor Xa cleaved
  • Calmodulin tag
  • S-tag
  • GFP protein tags
  • T7 epitope
    • MASMTGGQQMG (N terminal or internal)
    • Novagen T7 tag purification kit (Novagen), pGEMEX vectors (Promega)
    • Makrides 1996
  • V5 tag
    • GKPIPNPLLGLDST (C-terminal)
    • pET, pBAD and Gateway (Invitrogen)
    • McLean 2001

• protein solubility
  • maltose binding protein
  • Thioredoxin

• Protein cleavage sites [1]
  • Enterokinase
    • Asp-Asp-Asp-Asp-Lys↓
    • some side cutting at basic residues
  • Factor Xa
    • Ile-Glu/Asp-Gly-Arg↓
  • Thrombin
    • Leu-Val-Pro-Arg↓Gly-Ser
  • TEV protease
    • Glu-Asn-Leu-Tyr-Phe-Gln↓Gly
  • PreScission
    • Leu-Glu-Val-Leu-Phe-Gln↓Gly-Pro