Tk:protein tags

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  • protein purification tags, antibody epitopes
    • FLAG
    • c-MYC
      • EQKLISEEDL
    • HA
      • YPYDVPDYA
    • 6HIS
      • HHHHHH
    • Strep
      • AWRHPQFGG
      • Ala-Trp-Arg-His-Pro-Gln-Phe-Gly-Gly
    • GST (n-terminal)
    • Chitin binding
    • BCCP biotin carboxyl carrier protein
    • VSVG
    • Thrombin cleavage domain
      • LVPRGS (Leu-Val-Pro-Arg-Gly-Ser), factor Xa cleaved
    • Calmodulin tag
    • S-tag
    • GFP protein tags
    • T7 epitope
      • MASMTGGQQMG (N terminal or internal)
      • Novagen T7 tag purification kit (Novagen), pGEMEX vectors (Promega)
      • Makrides 1996
    • V5 tag
      • GKPIPNPLLGLDST (C-terminal)
      • pET, pBAD and Gateway (Invitrogen)
      • McLean 2001
  • protein solubility
    • maltose binding protein
    • Thioredoxin


  • Protein cleavage sites [1]
    • Enterokinase
      • Asp-Asp-Asp-Asp-Lys↓
      • some side cutting at basic residues
    • Factor Xa
      • Ile-Glu/Asp-Gly-Arg↓
    • Thrombin
      • Leu-Val-Pro-Arg↓Gly-Ser
    • TEV protease
      • Glu-Asn-Leu-Tyr-Phe-Gln↓Gly
    • PreScission
      • Leu-Glu-Val-Leu-Phe-Gln↓Gly-Pro
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