UA Biophysics:Protocols: Difference between revisions

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&bull; [[UA Biophysics:Protocols:Lipid Extraction|Lipid Extration]]<br>
&bull; [[UA Biophysics:Protocols:Lipid Extraction|Lipid Extration]]<br>
&bull; [[UA Biophysics:Protocols:Carotenoid Extration|Carotenoid Extration]]<br>
&bull; [[UA Biophysics:Protocols:Carotenoid Extration|Carotenoid Extration]]<br>
==Vesiculas gigantes==


==PLASMID==
==PLASMID==

Revision as of 15:14, 29 July 2015

BACTERIA STOCK

• Stocks

ANTIBIOTICS STOCK

• Ampicillin
• Kanamycin
• Chloramphenicol

MEDIA

• M9 Salts (5x) 1L
• M9 (5x) 1L
• LB 1L
• LB Plates 1L
• SD 1L
• 1M MgSO4
• 0.1M CaCl2

MOLECULAR BIOLOGY

• Q5 Polymerase

• Phusion Polymerase

EXTRACTION

• Lipid Extration
• Carotenoid Extration

Vesiculas gigantes

PLASMID

• GenEluteTM HP Plasmid Miniprep Kit
• Preparing Chemically Competent Cells
• Transforming Chemically Competent

MICROFLUIDICS

• Soft lithography
• Master fabrication
• Plasma Cleaner Protocol

TURBIDOSDOSTATS

• Plate turbidostat user manual
• Revolver turbidostat with interchanging media


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