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 Biomaterials Design Lab
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Objective
- Complete the procedure as outlined by Dr.Hartings here.<br.>
- Observe activity of pepsin and pepstatin in samples of hemoglobin. <br.>
- Observe activity using UV-Vis after certain intervals of time.<br.>
- At each measured interval take a sample of solution and stop the reaction to observe using SDS-PAGE. <br.>
Procedure
- Note that the hemoglobin had a concentration of 2nm in the pepsin solution.<br.>
- Note that the hemoglobin had a concentration of 20nm in the pepstatin solution. <br.>
Data
- This graph represents the absorbacne spectra of each hemoglobin and pepsin or hemoglobin and pepstatin solution at each time interval.
- It is worth noting that it is believed that the pepsin used wasn't active. This is because their wasn't a drastic difference between samples including pepsin and pepstatin.
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- Note that all of the samples were normalized by subtracting the signal of the buffer. Also the signals can be compared to the hemoglobin standard.
Biomaterials Design Lab
