User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2013/09/24

Biomaterials Design Lab

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Objective Complete the procedure as outlined by Dr.Hartings here.<br.> Observe activity of pepsin and pepstatin in samples of hemoglobin. <br.> Observe activity using UV-Vis after certain intervals of time.<br.> At each measured interval take a sample of solution and stop the reaction to observe using SDS-PAGE. <br.> Procedure Note that the hemoglobin had a concentration of 2nm in the pepsin solution.<br.> Note that the hemoglobin had a concentration of 20nm in the pepstatin solution. <br.> Data This graph represents the absorbance spectra of each hemoglobin and pepsin or hemoglobin and pepstatin solution at each time interval. It is worth noting that it is believed that the pepsin used wasn't active. This is because their wasn't a drastic difference between samples including pepsin and pepstatin.

• Note that all of the samples were normalized by subtracting the signal of the buffer. Also the signals can be compared to the hemoglobin standard.