User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2013/10/22: Difference between revisions

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==Objective==
==Objective==
*Synthesize Pepsin linked AuNP's.
*Synthesize Pepsin linked AuNP's as shown by Dr.Hartings [[User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/10/22|HERE]].
*Calculate the concentration ratio for pepsin/AuNP fiber formation.
*Calculate the concentration ratio for Pepsin/AuNP and HRP-AuNP fiber formation.


==Procedure==
==Procedure==
===Ratios were made as follows===
* These ratios allow a constant concentration of Gold at .25mM, and corresponding protein concentration.
* The amounts were measured using micropipettes.
* Stock Solutions: were as follows, Gold 2.52mM, HRP 15.3uM, and Pepsin 15.7 uM.
[[Image:Oct_22_ratios.png]]
*Once prepared, the test tubes were covered with foil and were baked for 4hrs at 80 degrees Celsius.


==Data==
==Data==
 
*No data was taken today!!
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__NOTOC__
__NOTOC__

Revision as of 17:42, 29 October 2013

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Objective

  • Synthesize Pepsin linked AuNP's as shown by Dr.Hartings HERE.
  • Calculate the concentration ratio for Pepsin/AuNP and HRP-AuNP fiber formation.

Procedure

Ratios were made as follows

  • These ratios allow a constant concentration of Gold at .25mM, and corresponding protein concentration.
  • The amounts were measured using micropipettes.
  • Stock Solutions: were as follows, Gold 2.52mM, HRP 15.3uM, and Pepsin 15.7 uM.

  • Once prepared, the test tubes were covered with foil and were baked for 4hrs at 80 degrees Celsius.

Data

  • No data was taken today!!


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