User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2013/11/13: Difference between revisions

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===HRP===
===HRP===
*The type of HRP we used in lab came from here;  
*The type of HRP we used in lab came from here;  
*[[http://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Datasheet/1/p8125dat.pdf| Product Data Sheet]]
*http://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma/Datasheet/1/p8125dat.pdf
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Revision as of 17:32, 18 November 2013

Biomaterials Design Lab <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Objective

  • Finish UV-Vis analysis on the Lysozyme samples with ratios ranging from 5:1 to 50:1 made on Nov 6th, 2013.
  • Finish UV-Vis analysis on the Hemoglobin samples with ratios ranging from 30:1 to 210:1 made on Nov 6th, 2013.
  • Discuss with group members about the final project and which three proteins we will be talking about.

Procedure

  • 1ml samples of the supernatant from each set of centrifuged nano-particles were used for UV-Vis analysis.
  • The large volumes of nano-particles were spun down and 1ml was used for UV-Vis analysis and the rest was used yesterday in the AA measurements.

Data

  • For the lysozyme AuNP's the absorbances make sense because these ratios extend both the areas where their are increasing concentrations of AuNP's and when the AuNP's formed fibers. As a result thats why both the low ratios and high ratios have low absorbances because either concentrations of AuNP's were low or its because fibers were formed.
  • For the hemoglobin AuNP's as the ratios increased the solutions progressively became darker and thus their was a steady increase of absorbance.

Project Ideas

  • We had decided that we will be talking about Lysozyme, HRP, and Myoglobin-linked AuNP's for the project.
  • Have decided to look at the structure of each of these proteins in order to try and understand the results we obtained over this past semester.

HRP


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