User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2014/04/01: Difference between revisions

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===Conductivity Measurements of 30:1 Lysozyme-AuNP at Room Temperature===
===Conductivity Measurements of 30:1 Lysozyme-AuNP at Room Temperature===
[[Image:4.1.conductivity.jac.png|800px|]]
[[Image:4.1.conductivity.jac.png|800px|]]
*PLEASE NOE THAT THESE CONDUCTIVITY MEASUREMENTS ARE NOTE CORRECTED FOR THE DILUTION FACTOR.
*PLEASE NOE THAT THESE CONDUCTIVITY MEASUREMENTS ARE NOT CORRECTED FOR THE DILUTION FACTOR.


===Atomic Absorption Preparation===
===Atomic Absorption Preparation===

Revision as of 20:41, 5 April 2014

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Objective

  • Run UV-Vis and AA on 30:1 lysozyme-AuNP samples from 3/25/14 (Only the 4 Degree Celsius samples were run).
  • Run conductivity on room temperature 30:1 lysozyme-AuNP samples from 3/25/14.

Procedure

Adding Variables to 30:1 Lysozyme-AuNP Synthesized 3/26/14

1. Add 2.25 mL of each variable to 4.5 mL of sample.

  • The variables used were:
  • 0.03 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0002316 M 2,2 bipyridine)
  • 0.06 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0004544 M 2,2 bipyridine)
  • 0.09 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0006772 M 2,2 bipyridine)

2. Put one of each sample in the following three environments: 4°C, 24°C, 36°C.


Conductivity Measurements of 30:1 Lysozyme-AuNP at Room Temperature

  • PLEASE NOE THAT THESE CONDUCTIVITY MEASUREMENTS ARE NOT CORRECTED FOR THE DILUTION FACTOR.

Atomic Absorption Preparation

Creating the Gold Stock Solutions

  1. Add 50 μL of HAuCl4·3H2O and 4950 μL of distilled water to a Falcon tube, for a final concentration of 10 μg/mL Au.
  2. Add 100 μL of HAuCl4·3H2O and 4900 μL of distilled water to a Falcon tube, for a final concentration of 20 μg/mL Au.
  3. Add 150 μL of HAuCl4·3H2O and 4850 μL of distilled water to a Falcon tube, for a final concentration of 30 μg/mL Au.
  4. Add 200 μL of HAuCl4·3H2O and 4800 μL of distilled water to a Falcon tube, for a final concentration of 40 μg/mL Au.
  5. Add 250 μL of HAuCl4·3H2O and 4750 μL of distilled water to a Falcon tube, for a final concentration of 50 μg/mL Au.


Atomic Absorption Samples

Solutions with the following Au:lysozyme ratio at room temperature were run on the AA:

  • 30:1 lysozyme-AuNP with 0.03 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0002316 M 2,2 bipyridine)
  • 30:1 lysozyme-AuNP with 0.06 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0004544 M 2,2 bipyridine)
  • 30:1 lysozyme-AuNP with 0.09 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0006772 M 2,2 bipyridine)



Adding Sodium Citrate to 30:1 Lysozyme-AuNP Samples

  1. Add 2.841 g of sodium citrate filled with deionized water to 10 mL in a 15 mL Falcon tube.
  2. Using serial dilutions, 1 mM, 10 mM, and 100 mM solutions were created.
  3. Take 1 mL aliquots from 30:1 lysozyme-AuNP samples and add 0.5 mL of each concentration of sodium citrate.


Figures

  • THIS DATA IS NO LONGER OF USE TO US. THIS IS BECAUSE THESE SAMPLES WERE PREPARED IN PLASTIC. THE RESULTS SEEN IN SAMPLE DON'T FOLLOW ANY OF THE TRENDS OBSERVED WHEN SAMPLES WERE MADE IN GLASS. WE DECIDED TO DISREGARD THESE RESULTS IN FAVOR OF LOOKING AT THE SAMPLES MADE IN GLASS.


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