User:Alexander Cvitan/Notebook/Experimental Biological Chemistry Lab/2014/04/02: Difference between revisions

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==Figures==
==Figures==
[[Image:4.2.14.aa.jac.png|850px|]]
[[Image:4.2.14.aa.jac.png|850px|]]
 
*AS SEEN IN THIS GRAPH MES IS THE ONLY VARIABLE THAT CAUSES AN INCREASE IN AMOUNT OF GOLD IN SOLUTION AS THE CONCENTRATION VARIABLE INCREASES. THIS WAS UNEXPECTED BECAUSE MES IN THE PAST HAS CAUSED FIBERS TO FALL OUT OF SOLUTION, AND THEY DID FALL OUT OF SOLUTION IN THIS CASE. ALSO CACL2 WAS SLIGHTLY ODD BECAUSE THE AMOUNT OF GOLD IN SOLUTION INCREASE FOR THE 60:1 VARIABLE. THE VARIABILITY COULD JUST BE DUE TO THE FACT THAT SO LITTLE GOLD IS IN SOLUTION THAT SLIGHT DIFFERENCES IN NEGLIGIBLE AMOUNTS ARE BEING OBSERVED. ALL OTHER VARIABLES FOLLOW THE EXPECTED TREND THAT [] OF GOLD DECREASES AS THE CONCENTRATION OF VARIABLE INCREASES.
===UV-VIS===
===UV-VIS===
[[Image:Screen_Shot_2014-04-05_at_11.07.43_PM.png]]
[[Image:Screen_Shot_2014-04-05_at_11.07.43_PM.png]]

Revision as of 20:24, 5 April 2014

Biomaterials Design Lab: Spring 2014 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Objective

  • Run UV-Vis and AA on room temperature 30:1 lysozyme-AuNP samples from 3/26/14.
  • Run conductivity on pure variables at room temperature.

Procedure

Conductivity Measurement of Pure Variables at Room Temperature


Atomic Absorption Preparation

Creating the Gold Stock Solutions

  1. Add 50 μL of HAuCl4·3H2O and 4950 μL of distilled water to a Falcon tube, for a final concentration of 10 μg/mL Au.
  2. Add 100 μL of HAuCl4·3H2O and 4900 μL of distilled water to a Falcon tube, for a final concentration of 20 μg/mL Au.
  3. Add 150 μL of HAuCl4·3H2O and 4850 μL of distilled water to a Falcon tube, for a final concentration of 30 μg/mL Au.
  4. Add 200 μL of HAuCl4·3H2O and 4800 μL of distilled water to a Falcon tube, for a final concentration of 40 μg/mL Au.
  5. Add 250 μL of HAuCl4·3H2O and 4750 μL of distilled water to a Falcon tube, for a final concentration of 50 μg/mL Au.


Atomic Absorption Samples

Solutions with the following Au:lysozyme ratio at room temperature were run on the AA:

  • 30:1 lysozyme-AuNP with 0.03 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0002316 M 2,2 bipyridine)
  • 30:1 lysozyme-AuNP with 0.06 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0004544 M 2,2 bipyridine)
  • 30:1 lysozyme-AuNP with 0.09 M MgCl2, CaCl2, NaCl, KCl, MES, citric acid (0.0006772 M 2,2 bipyridine)


Figures

  • AS SEEN IN THIS GRAPH MES IS THE ONLY VARIABLE THAT CAUSES AN INCREASE IN AMOUNT OF GOLD IN SOLUTION AS THE CONCENTRATION VARIABLE INCREASES. THIS WAS UNEXPECTED BECAUSE MES IN THE PAST HAS CAUSED FIBERS TO FALL OUT OF SOLUTION, AND THEY DID FALL OUT OF SOLUTION IN THIS CASE. ALSO CACL2 WAS SLIGHTLY ODD BECAUSE THE AMOUNT OF GOLD IN SOLUTION INCREASE FOR THE 60:1 VARIABLE. THE VARIABILITY COULD JUST BE DUE TO THE FACT THAT SO LITTLE GOLD IS IN SOLUTION THAT SLIGHT DIFFERENCES IN NEGLIGIBLE AMOUNTS ARE BEING OBSERVED. ALL OTHER VARIABLES FOLLOW THE EXPECTED TREND THAT [] OF GOLD DECREASES AS THE CONCENTRATION OF VARIABLE INCREASES.

UV-VIS

  • PLEASE NOTE, DUE TO THE LOW AMOUNT OF AUNP'S IN SOLUTION THE UNCERTAINTY IN THE INSTRUMENT PLAYS A SIGNIFICANT ROLE IN MEASUREMENTS ESPECIALLY SINCE THE PERCENTAGES ARE SO SMALL. AS A RESULT, THE TRENDS SHOULD BE TAKEN WITH A GRAIN OF SALT WHERE PERCENTAGES ARE VERY CLOSE.


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