User:Alexander S Mikheyev/Notebook/rotifer alien genes/2009/12/10
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 Alien genes
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Adineta Amp Primer 31, 32, 34 and AV10094F2+R2 (Primer 35) | |
| Plate | Well | Genus | 12/2/2009 Count | Sample | Extract | ' | Reagents | Concentration | 1X | 14X |
| AV 10-06-09 | well 6 | Adineta | 100+ | 2 | H20 | 4.75 | 66.5 | |||
| Boston | A1 | Adineta | 10+ | 3 | 10X Buffer | 10X | 1 | 14 | ||
| Boston | A4 | Adineta | 50+ | 4 | dye | 10X | 1 | 14 | ||
| CC | A1 | Adineta | subs 100+ | 5 | dNTP | 10mM | 0.2 | 2.8 | ||
| CC | A3 | Adineta | subs 100+ | 6 | primer mix | 5uM | 1 | 14 | ||
| WH1 | B3 | Adineta | 20+ | 7 | use 12/7 | Taq | 5U/uM | 0.05 | 0.7 | |
| WH1 | B5 | Adineta | 20+ | 8 | use 12/7 | DNA | variable | 2 | ||
| WH1 | B6 | Adineta | 50+ | 9 | use 12/7 | TOTAL | 10 | 112 | ||
| WH1 | C4 | Adineta | 30+ | 10 | Cocktail per well = 8.0ul | |||||
| WH2 | A6 | Adineta | 10+ | 11 | ||||||
| WH3 | A5 | Adineta | 20+ | 12 | On Gels | |||||
| WH3 | C2 | Adineta | 7 | 13 | Primers | AV10007 | #1-12 | |||
| AV10002 | #13-24 | |||||||||
| 4220F+7822R | #25-36 | |||||||||
| EXTRACTION | AV10094F2+R2 | #37-48 | ||||||||
| InstaGene Matrix | 200ul per sample | |||||||||
| 56C | 30 min | PCR | 95C | 3 min | ||||||
| 99.9C | 8min | 95C | 10 sec | |||||||
| 60C | 1 min | |||||||||
| 35 cycles | ||||||||||
| 12/2/2009 | 5-10 individuals | 12/7/2009 | 10-20 individuals | |||||||
| load 2.0ul 2-log ladder and 2.0ul sample 1% gel | ||||||||||
| Sample #2 Positive Control |
