User:Alicia Rasines Mazo/Notebook/CHEM-572 Experimental Biological Chemistry II/2015/01/27: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Tasks for January 27== | ==Tasks for January 27== | ||
* Formation of 30:1 Gold-Lysozyme nanoparticle colloid | * Formation of 30:1 Gold-Lysozyme nanoparticle colloid | ||
* Formation of 45:1 Gold- | * Formation of 45:1 Gold-Lysozyme fibers | ||
===Preparation of stock solutions=== | |||
*100 mL of 2.512mM HAuCl4 stock solution was prepared by adding 0.085 g of HAuCl4 in distilled water. | |||
**(0.1L)(2.512×10<sup>-3</sup> mol/L)(340 g/mol)=0.085 g (0.08533 g were weighed out) | |||
*50 mL of 20 μM lysozyme stock solution was prepared by adding 0.014307 g of Lysozyme in distilled water | |||
**((0.05L)(20×10<sup>-6</sup> mol/L)(14307 g/mol)= 0.014307 g (0.01417g were weighed out) | |||
Note: The pH of the solution has to be kept at 3.6 for optimum formation of AuNPs. | |||
===Preparation of Au colloid and fibers=== | |||
<u>30:1 AuLys colloid</u> | |||
*Per mL test tube, add 0.5 mL HAuCl<sub>4</sub> stock solution (final concentration 251 μM) | |||
* Also add 2.095 mL of 19.81μM of lysozyme stock | |||
**m<sub>1</sub>v<sub>1</sub>=m<sub>2</sub>v<sub>2</sub> | |||
**8.3×10<sup>-6</sup>(5mL)=19.81×10<sup>-6</sup>v<sub>2</sub> | |||
**v<sub>2</sub>=2.095 mL | |||
*Make up to 5 mL with distilled water (2.405 mL) | |||
*Repeat for 9 other test tubes | |||
<u>45:1 AuLys fibers</u> | |||
*Per mL test tube, add 0.5 mL HAuCl<sub>4</sub> stock solution (final concentration 251 μM) | |||
* Also add 1.40 mL of 19.81μM of lysozyme stock | |||
**m<sub>1</sub>v<sub>1</sub>=m<sub>2</sub>v<sub>2</sub> | |||
**5.56×10<sup>-6</sup>(5mL)=19.81×10<sup>-6</sup>v<sub>2</sub> | |||
**v<sub>2</sub>=1.40 mL | |||
*Make up to 5 mL with distilled water (3.1 mL) | |||
*Repeat for 9 other test tubes | |||
Place in oven for 4h at 80°C. | |||
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__NOTOC__ | __NOTOC__ |
Latest revision as of 00:41, 27 September 2017
Project name | Main project page Next entry |
Tasks for January 27
Preparation of stock solutions
Note: The pH of the solution has to be kept at 3.6 for optimum formation of AuNPs. Preparation of Au colloid and fibers30:1 AuLys colloid
45:1 AuLys fibers
Place in oven for 4h at 80°C. |