# User:Allison K. Alix/Notebook/Thesis Research/2013/04/09

(Difference between revisions)
 Revision as of 09:24, 12 April 2013 (view source) (→Data)← Previous diff Revision as of 09:39, 12 April 2013 (view source) (→Data)Next diff → Line 54: Line 54: Part 2: Part 2: + + [[Image:ThiolDNA_MB_pic_UV-Vis.png‎]] + + UV-Vis Spectrum of thiol-DNA/MB hybrid [[Image:ThiolDNA_MB_pic.png]] [[Image:ThiolDNA_MB_pic.png]] Line 60: Line 64: Part 3: Part 3: + + [[Image:ThiolDNA_ThT_pic_UV-Vis.png‎]] + + UV-Vis Spectrum of thiol-DNA/ThT hybrid [[Image:ThiolDNA ThT pic.png]] [[Image:ThiolDNA ThT pic.png]] + Fluorescence Spectrum of thiol-DNA/ThT hybrid Fluorescence Spectrum of thiol-DNA/ThT hybrid

## Revision as of 09:39, 12 April 2013

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## Objectives

• Take Abs and Fluorescence measurements of DNA-MB and DNA-ThT prepared on 4/4/13
• React ethane-thiol with AuNP

## Procedures

Part 1: Preparation of 1mL of 2.98μM ethane thiol

WARNING: ethanethiol is very volatile. Handle under the hood only wearing PPE.

• original concentration of AuNP = 19.4nM
• desired concentration of ethanethiol = 19.4nM x(15393) = 298624.2nM = 298μM
• density of ethanethiol = 0.839g/mL

1) Find the concentration of 0.0125mL pure ethanethiol in 5mL water

0.839g/mL x 0.0125mL = 0.0104875g

0.0104875g/(62.13 g/mol) = 1.68799 x 10-4 mol

1.68799 x 10-4 mol/5mL x (1000mL/1L) = 0.034M = 34mM

340mM (x) = (30mM)(1mL)

x = 0.088mL = 88μL in 912μL H2O

30mM (x) = 3mM (1mL)

x = 100μL in 900μL

3mM(x) = 298μM (1mL)

x = 99μL in 901μL to obtain 298μM ethanethiol

2) Reacting AuNP with ethanethiol

• react 200μL 298μM ethane thiol with 200μL 19.4nM AuNP (synthesized by Dr. Miller)

Part 2: Measure absorbance/fluorescence of AuNP-MB

Part 3: Measure absorbance/fluorescence of AuNP-ThT

## Data

Part 2:

UV-Vis Spectrum of thiol-DNA/MB hybrid

Fluorescence Spectrum of thiol-DNA/MB hybrid

Part 3:

UV-Vis Spectrum of thiol-DNA/ThT hybrid

Fluorescence Spectrum of thiol-DNA/ThT hybrid