User:Allison K. Alix/Notebook/Thesis Research/2013/09/10: Difference between revisions
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* If fluorescence is exhibited, run FCS measurements on dilutions | * If fluorescence is exhibited, run FCS measurements on dilutions | ||
==Addition of MB== | |||
[DNA] = 0.910μM, so [MB] must be 1.82μM | |||
Use 150μL of DNA-AuNP solution (10%=15μL) | |||
1.820x10-6M x 165x10-6L solution = 3.003x10-10 moles ThT | |||
(3.003x10-10 moles ThT)/ 15 x 10-6 L = 20.02μM | |||
-Add 15μL of 20.2μM to 150μL thiol-DNA/AuNP solution | |||
NEW Concentrations: | |||
[DNA]= 0.827μM, [ThT] = 1.82μM | |||
1) Prepare 100uL 20uM ThT (use already prepared 5/14/13) | |||
2) React with DNA ten minutes | |||
3) Observe absorbance and fluorescence | |||
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Revision as of 06:47, 10 September 2013
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To Do
Addition of MB[DNA] = 0.910μM, so [MB] must be 1.82μM Use 150μL of DNA-AuNP solution (10%=15μL) 1.820x10-6M x 165x10-6L solution = 3.003x10-10 moles ThT (3.003x10-10 moles ThT)/ 15 x 10-6 L = 20.02μM -Add 15μL of 20.2μM to 150μL thiol-DNA/AuNP solution NEW Concentrations: [DNA]= 0.827μM, [ThT] = 1.82μM 1) Prepare 100uL 20uM ThT (use already prepared 5/14/13) 2) React with DNA ten minutes 3) Observe absorbance and fluorescence | |
