User:Andrew K Farag/Notebook/CHEM-582/2015/02/18

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Objectives To place films from 02/13/2015 XRD molds into oven to cure To prepare a new batch of TiO2/epoxy and epoxy alone coatings Thaw NaaBlue Cells Grow cells Growth of cells NaaBlue Cells were taken from the -20 degrees Celsius freezer and thawed on ice for one hour Used all sterile supplies, (round bottom tubes and pipets) and ethanol to sterilize our gloved hands Open round bottom tube, open container of autoclaved broth and open plastic for pipet Transfer 5ml of autoclaved broth without touching any of the containers pipet should be not used more than once Torch the top portion of the autoclaved broth container and quickly torch the inside of the autoclaved broth top Close autoclaved broth container Dip metal loop into ethanol and torch, allow to cool for 30 seconds Open NaaBlue Cells Dip burned loop into cells and then dip the loop into the 5ml of broth and stir Torch the loop to kill off any bacteria Close up NaaCells and sore in freezer Solution should be cloudy with cells after 24 hours of incubation at room temperature.

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