Objectives
- Cure films from last lab period into the ovens
- Characterize swelling of epoxy films
- Prepare new films for PXRD analysis
- Autoclave broth solution
Measurement of swelling in epoxy film
- The film made on February 4 was cut into pieces, weighed and measured for thickness, then placed in water until the next lab period
Proposed Protocol for the Antimicrobial Activity of the film
- Grow the bacteria in broth in ambient temperature for two days/or till the solution becomes cloudy
- Pipette the bacteria/broth solution onto the film in petri dish
- Use a gauze patch to collect the solution from the film surface
- Place the gauze patch in an autoclaved NaCl/KCl solution
- Pipette solution onto a nutrient agar plate and spread over the surface
- agar plates should be incubated lid down, at 37C for 24 hours before colonies are counted
LB Broth Media
- measure 80 mL of water
- add 2 g of LB Broth
- autoclave and sterilize in 121C for 60 minutes, and leave for another hour until the pressure drops back to atmosphere pressure
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