User:Anthony Salvagno/Notebook/Research/2009/02/04/Lyticase

From OpenWetWare
< User:Anthony Salvagno‎ | Notebook‎ | Research‎ | 2009‎ | 02‎ | 04
Revision as of 08:11, 26 March 2009 by Anthony Salvagno (talk | contribs) (→‎Prep)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigationJump to search

This is the enzyme that does the spheroplasting job (Mark Wahlberg's next movie). (Steve Koch:I love your comments in these sections)

Prep

Need 10mL of solution:

  • Enzyme has 49xx units per gram
  • Measure out 10mg
  • add 10mL SB
  • separate into 400μL aliquots (made about 2524, I dropped one)