User talk:Anthony Salvagno
Re: Dunn Lab logo
- Ramalldf 20:03, 30 October 2009 (EDT):I made it a few months ago. Send it over, the reason that I used that font was cause I used the MASH font to make it look top secret for the private wiki logo so I just did the same but changed the colors.
Capping hairpin 2
Steve Koch 12:38, 11 August 2009 (EDT): OK, here's a google doc with my notes, and hopefully the same sequence as in the note below:
Here is the hairpin I used in grad school:
Unbiotinylated end-cap, HindIII 5’-P-TCGAGGCGACCTAGCGTAAGCTAGGTCGCC Name = P16-Cap-HindIII(incorrect!!!!) L=30 Purification = HPLC Modifications = 5’ phosphate for ligation
I think you should:
- Check with m-fold that it's a hairpin
- replace the 3 or 4 base sticky end with 3 or 4 based for SapI that you have
Andy Maloney 11:16, 7 May 2009 (EDT): So Larry thinks I'm nagging about the GRD monies. I know I am but, I just cannot stop thinking about what we should get. And, since we are getting just about everything for the Kiney experiments really quickly, I think we should act on this sooner rather than later. I know you said you wanted me and Larry to tell you what you should get so here is what we came up with.
- Model 250. Get the complete kit.
Actually, I found a more all purpose meter from VWR.
It has software that comes with it and it comes with 3 probes for pH, conductivity, and dissolved oxygen content. Evans has an osmometer but hey, this may work for us. I'll get a quote for the stuff.
We will need to get pH standards for the meter and possibly conductivity standards but CRLS has those. I have a quote for the water filter stuff that is not listed on VWR's site so you are left with about $150 which should cover shipping charges for this stuff.
Can you let me know when you will be in the lab next? I think we should have a Koch Lab meeting soon.
Recombinant Casein Paper
Andy Maloney 16:42, 24 April 2009 (EDT): So this is the paper I found that talks about making E. coli produce cow utter puss. I can't understand any of it but if we do end up using κ casein a lot, we may want to make our own. If you are up for a challenge and read this, can you explain it to me?