User:Anthony Salvagno/Notebook/Research/2010/12/17/Custom Plasmid for Unzipping/Stretching Construct: Difference between revisions
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(New page: {{qn}} I got the go ahead to get the DNA 2.0 plasmid. So I have to design oligo that will be embedded in the vector. ==Plasmid Design== ==PCR Design==) |
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==Plasmid Design== | ==Plasmid Design== | ||
I will get a 5kb vector from DNA 2.0 and have them add the sequence: | |||
:'''5' - CCAACGATCTGG''' | |||
which produces the overhang '''CGAT''', which is complementary to our adapter duplex. I'm allowed for anything up to 300bp (because it is the same price) so I'm pondering if I should add more too it or not. Right now I'm going with no and am requesting a quote. If I did add more what would it be? | |||
==PCR Design== | ==PCR Design== | ||
Revision as of 14:01, 17 December 2010
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I got the go ahead to get the DNA 2.0 plasmid. So I have to design oligo that will be embedded in the vector.
Plasmid Design
I will get a 5kb vector from DNA 2.0 and have them add the sequence:
- 5' - CCAACGATCTGG
which produces the overhang CGAT, which is complementary to our adapter duplex. I'm allowed for anything up to 300bp (because it is the same price) so I'm pondering if I should add more too it or not. Right now I'm going with no and am requesting a quote. If I did add more what would it be?
