User:Anthony Salvagno/Notebook/Research/2010/12/17/Custom Plasmid for Unzipping/Stretching Construct: Difference between revisions
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Once I get a quote I will ask for the vector sequence so that I can plan the PCR reaction. | Once I get a quote I will ask for the vector sequence so that I can plan the PCR reaction. | ||
The vector sequence can be found here: | The vector sequence can be found here:<br /> | ||
[[/BstXI Custom Plasmid Sequence]] - this thing should have a cool name. How about Plasmid Destroyer 8003? I could name it Vector Sigma in honor of transformers, but that is gay. I know, IheartPlasmid... yea! | [[/BstXI Custom Plasmid Sequence]] - this thing should have a cool name. How about Plasmid Destroyer 8003? I could name it Vector Sigma in honor of transformers, but that is gay. I know, IheartPlasmid... yea! | ||
==PCR Design== | ==PCR Design== | ||
Revision as of 14:03, 17 December 2010
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I got the go ahead to get the DNA 2.0 plasmid. So I have to design oligo that will be embedded in the vector.
Plasmid Design
I will get a 5kb vector from DNA 2.0 and have them add the sequence:
- 5' - CCAACGATCTGG
which produces the overhang CGAT, which is complementary to our adapter duplex. I'm allowed for anything up to 300bp (because it is the same price) so I'm pondering if I should add more too it or not. Right now I'm going with no and am requesting a quote. If I did add more what would it be?
Once I get a quote I will ask for the vector sequence so that I can plan the PCR reaction.
The vector sequence can be found here:
/BstXI Custom Plasmid Sequence - this thing should have a cool name. How about Plasmid Destroyer 8003? I could name it Vector Sigma in honor of transformers, but that is gay. I know, IheartPlasmid... yea!
